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Course Description Course Name : PAGE Tecniques Course Code : BY-707 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. HATİCE KORKMAZ GÜVENMEZ Learning Outcomes of the Course : Has a clear conception gel electrophoresis techniques in molecular biology applications. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : To make an inquiry about PAGE techniques as theoretical and practical Course Contents : Preparation for protein isolation (preparation of buffers, effects of buffer concentrations on pH, preventing buffer contamination, salts, metal ions and chelators, inhibitors, storage, temperature etc. Reducing agents Detergents Protein solubilization Proteas inhibitors Gel electrophoresis under denaturing conditions, Gradient gels, SDS-Urea gels, Molecular weight determination, Protein Quantitation (densitometry), Gel electrophoresis under nondenaturing conditions, Isoelectric focusing and 2D gel electrophoresis. Language of Instruction : Turkish Work Place : laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Preparation for protein isolation (preparation of buffers) scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 2 Effects of buffer concentrations on pH scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 3 Preventing buffer contamination scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 4 salts, metal ions and chelators, inhibitors, storage, temperature etc. scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 5 Reducing agents and detergents scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion , practise 6 Protein solubilization scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 7 Protease inhibitors scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion , practise 8 mid-term exam 9 gel electrophoresis under denaturing conditions scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion , practise 10 Gradient gels scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 11 SDS-urea gels scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 12 Molecular weight determination, scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 13 Protein Quantitation (densitometry), scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 14 Gel electrophoresis under nondenaturing conditions, scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 15 Isoelectric focusing and 2D gel electrophoresis. scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion, practise 16/17 final exam final exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Protein Methods, Bollag and Edelstein, 2000 Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 80     Homeworks/Projects/Others 1 20 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has the advanced hypothetical and applied knowledges in biology field 4 2 Based on the bachelor´s level of qualification, develops and deepens the knowledge as a specialist in the biology 4 3 Uses the theoretical and practical knowledge he expertises in biology 3 4 Integrates and interprets the knowledge from different disciplines by his expertise in biology and generates new information. 4 5 Solves the problems of biology by using research methods. 3 6 Carries out an independent study requires expertise on biology 3 7 Develops and produces new strategic approaches and solves the unexpected problems in biology area by taking responsibilty 5 8 Assesses the knowledge he expertises through critical approach and makes leadership solving problems related to biological environments. 3 9 Systematically transfers the current developments in the field of biology and his/her studies to the person in biology field or other related fields by supporting qualitative and quantitative data 4 10 Uses at least one foreign language to keep up to date with the field to take part in scientific and social interaction groups to share information at international levels 3 11 Uses information and communication technologies as required in the biology field 1 12 In biology, uses statistical software programme to carry out scientific research and also uses information and communication technology in proficiency level of knowledge and skills to critically evaluate 2 13 Develops the strategy, policy and implementation plans in the issues related to the field of biology and treats the results obtained within standart of quality 3 14 Uses the knowledge, problem solving, and / or practical skills obtained in the Biology Field in interdisciplinary studies. 4 15 Oversees social, scientific, cultural and ethical values in order to collect, implement, interpret data in the Biology area 5 16 Has the ability to formulate and solve the problems of biology in advanced level 4 17 Has ability to use his advanced knowledge in biology field for thinking systematically and solve problems in multidisciplinary approaches 4 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 15 2 30     Out of Class Study (Preliminary Work, Practice) 15 2 30 Assesment Related Works     Homeworks, Projects, Others 1 20 20     Mid-term Exams (Written, Oral, etc.) 1 30 30     Final Exam 1 30 30 Total Workload: 140 Total Workload / 25 (h): 5.6 ECTS Credit: 6