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Course Description Course Name : Molecular Biology Course Code : BL 361 Course Type : Compulsory Level of Course : First Cycle Year of Study : 3 Course Semester : Fall (16 Weeks) ECTS : 3 Name of Lecturer(s) : Prof.Dr. BURHAN ARIKAN Learning Outcomes of the Course : Learn gene cloning and recombinant DNA techniques and their application Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : BL 361 Molecular Biology Recommended Optional Programme Components : None Aim(s) of Course : To teach nucleic acid in different microorganism, mobile genetic materials and their isolation, gene cloning, recombinant DNA methods, PCR and their applications Course Contents : Introductions to Molecular Biology, Plasmids and Bacteriophages, Isolation of Plasmid and Bacteriophages nucleic acids, properties and impotance of transposane and transposable structural, DNA isolation of animal and plant cells, The mechanisms of natural genetic material of microorganisms and plant cells, transformation (CaCL2, RbCL2, Protoplast and Liposome) electroporation, electrofusion, biolistic transformation, gene cloning, ploymerase chain reaction, hybridization (southern, northern, ın situ and dot blot), cloning vectors, recombinant DNA production and transfer to recipient cell, molecular diagnostic methods (DNA fingerprint, RFLP, 16S ribosomal RNA genes, RAPD) Language of Instruction : Turkish Work Place : Class   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Introductions to Molecular Biology Study in literature Oral presentation 2 Plasmids and Bacteriophages, Isolation of Plasmid and Bacteriophages nucleic acids, Study in literature Oral presentation 3 properties and impotance of transposane and transposable structural Study in literature Oral presentation 4 DNA isolation of animal and plant cells Study in literature Oral presentation 5 The mechanisms of natural genetic material of microorganisms and plant cells Study in literature Oral presentation 6 Conjugation, electroporation, electrofusion, biyolistic transformation Study in literature Oral presentation 7 Exam 8 transformation (CaCL2, RbCL2, Protoplast and Liposome) Study in literature Oral presentation 9 gene cloning and cloning techniques Study in literature Oral presentation 10 PCR Study in literature Oral presentation 11 Hibridisation (Souther, northern, In situ, Dot bloth) Study in literature Oral presentation 12 cloning vectors Study in literature Oral presentation 13 recombinant DNA production and transfer to recipient cell Study in literature Oral presentation 14 Isolation of recombinant organisms Study in literature Oral presentation 15 Hibridoma and cell fusion techniques Study in literature Oral presentation 16/17 Final exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)         Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 40     Homeworks/Projects/Others 3 60 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Develops his/her professional experiences and knowledges by taking elective courses together with basic subjects of biology 2 2 takes responsibility as an individual or in a group to solve unpredicted problems that would be encountered 2 3 plans and manages the projects as a member of team 2 4 informs an indivudual and institutions about the subjects related to biology, and prepare verbal and written presentation to solve the problems 3 5 communicates effectively with colleagues in the world about biological issues and works 1 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 2 28     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 3 1 3     Mid-term Exams (Written, Oral, etc.) 1 8 8     Final Exam 1 20 20 Total Workload: 87 Total Workload / 25 (h): 3.48 ECTS Credit: 3