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  Course Description
Course Name : Plankton Culture

Course Code : SUF304

Course Type : Compulsory

Level of Course : First Cycle

Year of Study : 3

Course Semester : Spring (16 Weeks)

ECTS : 4

Name of Lecturer(s) : Prof.Dr. OYA IŞIK

Learning Outcomes of the Course : Counts the physical and chemical condiitions required for the phytoplanktonic organisms.
Explain the reasons for culturing microalgae, provides brief information on the history of the first species cultured.
Counts the number of necessary conditions in the laboratory, batch and continuous culture defines and explains the methods.
Defines sterilization, counts sterilization methods and explain the principles, outlined how it works, describes the equipment used for sterilization.
Describes the cultures of algae growth, makes the definition of growth, defines the growth parameters, makes the analysis of chlorophyll a, counts cell, measures optical density of the culture.
Counts the microalgal isolaion methods and explains them.
Defines the continuous and batch culture, prepare the growt medium.
Defines the isolations techniques of microalgae, prepare the solid medium using agar, makes planting.
The selection criteria will be cultured algae, microalgae species widely from culture,
Describes the general principles of the outdoor culture, the hotobioreactors and the ponds.
Makes chlorophyll a analysis, count cell, measures optic density of the culture, makes dry matter of analysis
Explain the rotifer culture, describes the rotifer culture conditions.
Makes chlorophyll a analysis, count cell, measures optic density of the culture, makes dry matter of analysis
Explains Artemia culture conditions and hatching artemia cysist.
Defines the Daphnia culture conditions.

Mode of Delivery : Face-to-Face

Prerequisites and Co-Prerequisites : None

Recommended Optional Programme Components : None

Aim(s) of Course : To culture phytoplankton in laboratory and outdoor, open and closed systems, to supply the reguired physical and chemical conditions to culture phytoplankton and zooplankton, to determine the phytoplankton ve zooplankton species and their basic biological needs. To define culture methods, microalgal isolation methods, growth and measure. Prepare the growth medium and inoculate to the culture. Harvest the phytoplankton and zooplankton, know the harvesting methods.

Course Contents : Culture conditions of phytoplanktonic organisms, continuous and batch culture methods, microalgal culture and sterilisation, microalgae isolation techniques, laboratory conditions, physical and chemical conditions necessary for the culture of microalgae, description of algal growth, parameters of algal growth and mesurements, the medium preparation and nutrients, inoculations, harvesting, microalgal culture in the ponds outdoor, description of photobioreactor, zooplankton culture, phytoplankton and zooplankton species cultured.

Language of Instruction : Turkish

Work Place : Fisheries Faculty Classrooms, Algal Biotechnology Laboratory, Photobioreactors and Ponds


  Course Outline /Schedule (Weekly) Planned Learning Activities
Week Subject Student's Preliminary Work Learning Activities and Teaching Methods
1 History of plankton culture, reasons of microalgal culture, physical and chemical requirements of microalgae. Introduction to the course, supplying the lecture notes. Face to face lecture, questions and answers
2 Plankton culture laboratory conditions, Introduction to laboratory, light, temperature, aeration-mixing, nutrients and preparing nutrient medium. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers
3 Definition of sterilization, sterilization methods, sterilization of the nutrient medium, the sample preparation of nutrient medium. Come prepared lecture notes and lab sheet. Face to face lecture, weight the nutrient in laboratory, filter sea water, prepare nutrient medium and autoclave..
4 Description of the growth of algae cultures, aAlgal growth stages, growth parameters, cell count and measurement of growth. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers, under the microscope cell counts.
5 Microalgae culture methods, batch and continuous culture methods, determination of dry matter analysis of algal growth. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers, do the dry matter analysis in laboratory.
6 Microalgae isolation methods. Chlorophyll a analysis. Come prepared lecture notes and lab sheet. Do the homework of dry matter calculation. Face to face lecture, questions and answers, do the analysis of chlorophyll a.
7 Widely cultivated species of algae, the physical and chemical requirements, microscopic examination of the species. Come prepared lecture notes and lab sheet. Do the homework of chlorophyll a calculation. Face to face lecture, questions and answers, Examine the species of microalgae under the microscope.
8 Midterm exam Study for the Exam Midterm exam
9 Outdoor algae culture, general principles of microalgae cultures in the ponds and photobioreactors. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Examination of the ponds and the photobioreactors.
10 Microalgal product harvesting, optic density measurement. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Measure the optical density of the culture by taking an example.
11 Preparing of nutrient medium for ponds and photobioreactors and inoculations. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Prepare the nutrient medium and inoculate the mediums.
12 Rotifer culture, the necessary conditions for the culture of rotifers. Morphological structure of the rotifer. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Examine the rotifer under the microscop.
13 Daphnia culture, the necessary conditions for the culture of Daphnia. Morphological structure of Daphnia. Feeding Rotifer and Daphnia. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Examine the Daphnia under the microscop. Feed Rotifer and Daphnia.
14 Artemia culture, to prepare the physical and chemical conditions for hatching the Artemia cyst and hatching the cyst. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Artemia cyst are hatching.
15 Morphological structure of incubated Artemia and feeding them. Come prepared lecture notes and lab sheet. Face to face lecture, questions and answers. Feed the Artemia naupliiActive lec and examine under the microscop.
16/17 Final examination. Study for the Exam Final examination.


  Required Course Resources
Resource Type Resource Name
Recommended Course Material(s)  Prepared lecture notes
 A. Richmond (1986), CRC Handbook of Microalgal Mass Culture,Taylor&Francis, p: 528.
 Laboratory sheets
 G. E., Fogg and B. Thake (1987), Algal Cultures and Phytoplankton Ecology, Printed in USA, p:259.
 Cirik S., Gökpınar Ş., 2005. Plankton and Culture, Printed in Ege Üniv. , İZMİR.
Required Course Material(s)


  Assessment Methods and Assessment Criteria
Semester/Year Assessments Number Contribution Percentage
    Mid-term Exams (Written, Oral, etc.) 1 70
    Homeworks/Projects/Others 2 30
Total 100
Rate of Semester/Year Assessments to Success 40
 
Final Assessments 100
Rate of Final Assessments to Success 60
Total 100

  Contribution of the Course to Key Learning Outcomes
# Key Learning Outcome Contribution*
1 Using the informatics and communicating technology 3
2 Gaining competence to determine the current status of aquatic resources and its sustainable use, water pollution and control, and biotechnology areas. 4
3 Ability to act in accordance with the regulation, social, scientific, cultural, and ethical values on fisheries field 5
4 Having knowledge on “natural and applied sciences” and “basic engineering”; combination of their theoretical and practical knowledge on fisheries engineering applications. 4
5 assessment of data scientifically on fisheries engineering, determining and solving the problems 5
6 Uses theoretical and practical knowledge in the field of fisheries to design; investigates and interprets events and phenomena usig scientific methods and techniques. 4
7 Collecting data in fisheries science, making the basic experimental studies, evaluating the results, identifying the problems and developing methods of solution 4
8 Having plan any study related to fisheries science as an individually, managing and consulting 5
9 Learning the knowledge by the determining learning needs; developing positive attitude towards lifelong learning 4
10 Communicating oral and written in expertise field, monitoring the seminars and meeting in expertise field, following the foreign language publication 5
11 Improving life-long learning attitude and using the information to the public interest. 4
12 Having skills to apply modern techniques and computational tools necessary for engineering applications. 4
13 Having ability to promote the study about aquaculture techniques by saving the natural environment, fishery diseases, fishing and processing technology, structure of fishery sector, problems and solution of their expertise field 5
14 Having ability to promote the study about aquaculture techniques by saving the natural environment, fishery diseases, fishing and processing technology, structure of fishery sector, problems and solution of their expertise field 4
15 Improves constantly itself , as well as professional development scientific, social, cultural and artistic fields according to his/her interests and abilities identifying needs of learning. 0
* Contribution levels are between 0 (not) and 5 (maximum).

  Student Workload - ECTS
Works Number Time (Hour) Total Workload (Hour)
Course Related Works
    Class Time (Exam weeks are excluded) 14 3 42
    Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
    Homeworks, Projects, Others 2 3 6
    Mid-term Exams (Written, Oral, etc.) 1 1 1
    Final Exam 1 1 1
Total Workload: 106
Total Workload / 25 (h): 4.24
ECTS Credit: 4