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Course Description Course Name : Ultra-Cold Conditions (Cryopreservation) Preservation Methods and Applications Course Code : BB-625 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. YILDIZAKA KAÇAR Assoc.Prof.Dr. NESLİHAN YEŞİM YALÇIN MENDİ Learning Outcomes of the Course : Describes the development and basis of cryopreservation. Learns the cryopreservation techniques. Learns the protection of plant materials for biotechnological methods Learns cryopreservation and thawing processes. Learns the properties of cryoprotectants and vitrification, desiccation techniques. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : This course aims to teach the basic concepts and subjects of cryopreservation. Course Contents : The contents of this course include introduction to the historical development of cryopreservation techniques; slow cooling techniques; rapid cooling techniques; cold hardening, cryoprotectants (DMSO, ethylene glycol, sucrose, mannitol, sorbitol, etc.); vitrification technique and the preparation of vitrification solutions (PVS1, PVS2, PVS3, etc.); desiccation technique (silica gel and laminar flow cabinet); synthetic seed production (encapsulation); encapsulation dehydration; desiccation encapsulation; droplet method. Language of Instruction : Turkish Work Place : Classrooms and Biotechnology labs of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 The historical development of cryopreservation techniques Reading the related lecture notes Straight narrative technique, power point presentation and discussion 2 Slow cooling techniques Reading the related lecture notes Straight narrative technique, power point presentation and discussion 3 Rapid cooling techniques Reading the related lecture notes Straight narrative technique, power point presentation and discussion 4 Cold hardening Reading the related lecture notes Straight narrative technique, power point presentation and discussion 5 Cryoprotectans Reading the related lecture notes Straight narrative technique, power point presentation and discussion 6 Vitrification technique and preparation of solutions Reading the related lecture notes Straight narrative technique, power point presentation and discussion 7 Desiccation technique Reading the related lecture notes Straight narrative technique, power point presentation and discussion 8 Production of synthetic seeds Reading the related lecture notes Straight narrative technique, power point presentation and discussion 9 Encapsulation dehydration Reading the related lecture notes Straight narrative technique, power point presentation and discussion 10 Encapsulation desiccation Reading the related lecture notes Straight narrative technique, power point presentation and discussion 11 Encapsulation vitrification Reading the related lecture notes Straight narrative technique, power point presentation and discussion 12 Cryopreservation of embriyogenic culture Reading the related lecture notes Straight narrative technique, power point presentation and discussion 13 Cryopreservation of Bryophytes and fern Reading the related lecture notes Straight narrative technique, power point presentation and discussion 14 Droplet method Reading the related lecture notes Straight narrative technique, power point presentation and discussion 15 Final Exam Preparation to the final exam Writen exam 16/17 General review Reading the related lecture notes Straight narrative technique, power point presentation and discussion   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Plant Cryopreservation: A Practical Guide (Ed. Barbara M. Reed)- Cryopreservation Practical Considerations (Barbara M. Reed)- Cryopreservation Theory (Erica E. Benson) Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 90     Homeworks/Projects/Others 2 10 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 3 2 Understanding the horticulture and the interaction between the related disciplines 2 3 Solving problems by research methods and establishing cause-effect relationship 4 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 4 5 Carrying out a study individually that requires professional knowledge in Horticulture 4 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 3 7 Leading in the situations when solving problems related to horticulture is necessary 2 8 Entering database related to horticulture, utilizing those sources and self-updating. 3 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 4 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 2 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 2 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 2 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 4 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 20 1 20 Assesment Related Works     Homeworks, Projects, Others 2 15 30     Mid-term Exams (Written, Oral, etc.) 1 15 15     Final Exam 1 22 22 Total Workload: 143 Total Workload / 25 (h): 5.72 ECTS Credit: 6