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Course Description Course Name : Bacterial Plasmids Course Code : BY-607 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. HATİCE KORKMAZ GÜVENMEZ Learning Outcomes of the Course : Understands the bacterial plasmids and their importance and using of them as the genetic vectors Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : BY-607 Bacterial Plasmids Recommended Optional Programme Components : None Aim(s) of Course : To know extrachromosomal genetic materials and their importance Course Contents : Plasmid replication and maintenance Single-cell resistance (SCR) to antibiotics Determination of plasmid copy number Imcompatibility Basic replicons, origins of replication Plasmids from gram positive bacteria Classification Isolation of plasmid DNA Host range and plasmid transfer Lactococcal plasmid vectors Lactococcal vector systems Virulence plasmids Isolation of plasmids (Large and small scale) Plasmid curing Streptomyces plasmid vectors Autonomously replicating plasmid vectors Phagemids and other hybrid vectors Language of Instruction : Turkish Work Place : my office or classroom   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Plasmid replication and maintenance scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 2 Single-cell resistance (SCR) to antibiotics scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 3 Determination of plasmid copy number scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 4 Imcompatibility scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 5 Basic replicons, origins of replication scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 6 Plasmids from gram positive bacteria scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 7 Classification Isolation of plasmid DNA Host range and plasmid transfer scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 8 mid-term exm 9 Lactococcal plasmid vectors scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 10 Lactococcal vector systems scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 11 Virulence plasmids scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 12 Isolation of plasmids (Large and small scale), plasmid curing scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 13 Streptomyces plasmid vectors scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 14 Autonomously replicating plasmid vectors scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion telling with lecture notes, presentation with projection, and interactive discussion 15 Phagemids and other hybrid vectors scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 16/17 Final exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Plasmids, A Practical Approach, hardy, K.G., 1998 Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 50     Homeworks/Projects/Others 5 50 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has the advanced hypothetical and applied knowledges in biology field 3 2 Based on the bachelor´s level of qualification, develops and deepens the knowledge as a specialist in the biology 4 3 Uses the theoretical and practical knowledge he expertises in biology 4 4 Integrates and interprets the knowledge from different disciplines by his expertise in biology and generates new information. 4 5 Solves the problems of biology by using research methods. 5 6 Carries out an independent study requires expertise on biology 4 7 Develops and produces new strategic approaches and solves the unexpected problems in biology area by taking responsibilty 3 8 Assesses the knowledge he expertises through critical approach and makes leadership solving problems related to biological environments. 4 9 Systematically transfers the current developments in the field of biology and his/her studies to the person in biology field or other related fields by supporting qualitative and quantitative data 3 10 Uses at least one foreign language to keep up to date with the field to take part in scientific and social interaction groups to share information at international levels 4 11 Uses information and communication technologies as required in the biology field 1 12 In biology, uses statistical software programme to carry out scientific research and also uses information and communication technology in proficiency level of knowledge and skills to critically evaluate 1 13 Develops the strategy, policy and implementation plans in the issues related to the field of biology and treats the results obtained within standart of quality 3 14 Uses the knowledge, problem solving, and / or practical skills obtained in the Biology Field in interdisciplinary studies. 5 15 Oversees social, scientific, cultural and ethical values in order to collect, implement, interpret data in the Biology area 3 16 Has the ability to formulate and solve the problems of biology in advanced level 4 17 Has ability to use his advanced knowledge in biology field for thinking systematically and solve problems in multidisciplinary approaches 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 2 28     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 5 5 25     Mid-term Exams (Written, Oral, etc.) 1 20 20     Final Exam 1 40 40 Total Workload: 141 Total Workload / 25 (h): 5.64 ECTS Credit: 6