DEGREE PROGRAMS  Associate's Degree (Short Cycle)  Bachelor’s Degree (First Cycle)  Master’s Degree (Second Cycle)

Course Description Course Name : Hybridization Breeding in Citrus Course Code : BB-584 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Assoc.Prof.Dr. BİLGE YILMAZ Prof.Dr. TURGUT YEŞİLOĞLU Learning Outcomes of the Course : Knows classical and somatic hybridization in citrus scion and rootstock breeding Knows enzymatic and mechanical protoplast isolation methods and protoplast fusion techniques Gains theorical and practical knowledge on related research techniques Gains skills for applying research results and theorical knowledge to practice Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aims this course are to teach classical and somatic hybridization techniques in citrus; citrus flower biology, breeding strategy and aspects of hybridization breeding; important factors affecting the success of breeding; structure of the cell wall; protoplast isolation techniques (mechanical and chemical); explant resources, washing solutions used in enzymatic isolation; enzymes and their mixtures, washing and purifying the protoplast; determining the size and efficiency of protoplast; protoplast fusion methods (chemical, mechanical and electro-fusion); protoplast fusion and culturing the fused cells. Course Contents : The main objectives of this course are classical and somatic hybridization techniques in citrus; citrus flower biology, breeding strategy and aspects of hybridization breeding; important factors affecting the success of breeding; possible usage of somatic hybridization in regards to scion and rootstock breeding, hybridization breeding process, solutions for the problems in hybridization breeding. Language of Instruction : Turkish Work Place : Classroom and citrus orchards of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 General information about classical and somatic hybridization techniques in citrus Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 2 Flower structure and reproductive biology in citrus Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 3 Breeding strategy and the importance of hybridization breeding in citrus Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 4 Scion breeding by classical hybridization in citrus Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 5 The aspects and important points in orange and mandarin variety development Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 6 The aspects and important points in lemon and grapefruit variety development Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 7 Mid-term exam Preparation to the mid-term exam Writen exam 8 Rootstock breeding by classical hybridization in citrus Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 9 Protoplast isolation, aims and scopes of application Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 10 Mechanic and enzymatic methods in protoplast isolation Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 11 Explant resources, growing conditions of donor plant, washing and enzym solutions in protoplast isolation Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 12 Washing, purifying and measuring the size and efficiency of protoplasts. Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 13 Protoplast fusion methods (chemical, mechanic and electrofusion), protoplast fusion and conditions for culturing the fused cells Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 14 Possible usages of classical and somatic hybridization methods in scion breeding Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 15 Possible usages of classical and somatic hybridization methods in rootstock breeding Reading related chapters of recommended resources. Straight narrative technique, power point presantation and discussion 16/17 Final exam Preparation to the final exam Writen exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Notes prepared by using different sources and transferred to electronic media, presentation, enriched with photos  - Citrus, Genetics, Breeding and Biotechnology, Edited by I. Khan - Tropical Plant Breeding, Editors: Andre Charrier, Michel jacquot, Serge hamon and Dominique Nicolas - Breeding Tropical and Subtropical Fruits. P.K. Ray Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 100     Homeworks/Projects/Others 0 0 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 4 2 Understanding the horticulture and the interaction between the related disciplines 3 3 Solving problems by research methods and establishing cause-effect relationship 2 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 3 5 Carrying out a study individually that requires professional knowledge in Horticulture 2 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 3 7 Leading in the situations when solving problems related to horticulture is necessary 3 8 Entering database related to horticulture, utilizing those sources and self-updating. 1 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 1 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 3 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 3 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 4 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 1 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 5 70 Assesment Related Works     Homeworks, Projects, Others 0 0 0     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 25 25 Total Workload: 147 Total Workload / 25 (h): 5.88 ECTS Credit: 6