DEGREE PROGRAMS  Associate's Degree (Short Cycle)  Bachelor’s Degree (First Cycle)  Master’s Degree (Second Cycle)

Course Description Course Name : Introduction to Cell Culture Course Code : HİS514 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 4 Name of Lecturer(s) : Asst.Prof.Dr. HÜLYA ÖZGÜR Prof.Dr. SAİT POLAT Learning Outcomes of the Course : Identifies the equipments required for a cell culture laboratory. Learns the rules of the cell culture laboratory. Understands the fundamental cell culture techniques. Applies basic cell culture methods. Solves the problems in the cell culture laboratory. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : HİS512 Techniques of Light Microscopy I Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is learning the cell culture technics and applying them. Course Contents : Cell culture types,Carbondioxide incubators, Microscope, Preservation conditions, Cell culture storages, Cell production, Cell culture media, Cell culture security Language of Instruction : Turkish Work Place : Laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 What is cell culture and the purpose of it? Self-study, reading recommended soruces Presentation 2 Historical events in development of the cell culture Self-study, reading recommended soruces Presentation 3 Types of cell cultures Self-study, reading recommended soruces Presentation 4 The usage of sub-culture method Self-study, reading recommended soruces Presentation 5 Culture mediums Self-study, reading recommended soruces Presentation 6 Culture Dishes Self-study, reading recommended soruces Presentation and demostration 7 The usage of incubators Self-study, reading recommended soruces Presentation and demostration 8 Midterm exam Self-study, reading recommended soruces Written Exam 9 Cell culture contamination Self-study, reading recommended soruces Presentation 10 Basic aseptic conditions Self-study, reading recommended soruces Presentation 11 Safety precautions of the cell culture Self-study, reading recommended soruces Presentation 12 Freezing of the cells for storage Self-study, reading recommended soruces Presentation and demostration 13 Working with cryopreserved cells Self-study, reading recommended soruces Presentation 14 Cell viability Self-study, reading recommended soruces Presentation 15 Transfection methods Self-study, reading recommended soruces Presentation 16/17 Final Self-study, reading recommended soruces Written Exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Davis JM.: Basic cell culture. A practical approach. Oxford University press. 1994, New York.  Masters JRM.: Animal cell culture. 3rd ed. Oxford University press. 2005. Required Course Material(s)  Lecture powerpoint presentations   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 100     Homeworks/Projects/Others 1 0 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Tell the main histological features of cells and tissues. 0 2 Corelate the structure and function of cells. 0 3 Have knowledge of the histologic features of organ systems. 0 4 Have knowledge of embryo development, developmental disorders and the mechanisms. 0 5 Define the methods of light and electron microscopic examination and tissue preparation. 0 6 Have knowledge of general principles about cell culture, histochemistry and immunohistochemistry. 5 7 Prepare solutions and tissues for light and electron microscopic studies . 3 8 Apply the basic methods of staining. 0 9 Distinguish tissue and organ systems in light microscopic level. 0 10 Perform studies as a team member or indıvidual. 0 11 Perform tasks which is given in research projects. 0 12 Develop positive attitudes and behaviors towards lifelong learning. 0 13 Determine and direct needs and resources of learnings by using resources effectively. 0 14 Evaluate knowledges and skills of the master degree critically. 0 15 Communicate effectively. 0 16 Relay ideas and solution offers about current developments in studies by supporting them with quantitative and qualitative data visually and verbally. 0 17 Know the importance of the ethical principles in research studies, evaluations and results. 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 1 2 2     Mid-term Exams (Written, Oral, etc.) 1 2 2     Final Exam 1 2 2 Total Workload: 90 Total Workload / 25 (h): 3.6 ECTS Credit: 4