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| Course Description |
|
| Course Name |
: |
Biochemistry Lab II |
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| Course Code |
: |
KM 438 |
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| Course Type |
: |
Compulsory |
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| Level of Course |
: |
First Cycle |
|
| Year of Study |
: |
4 |
|
| Course Semester |
: |
Spring (16 Weeks) |
|
| ECTS |
: |
2 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. SEYHAN TÜKEL
|
|
| Learning Outcomes of the Course |
: |
Learn biochemical analysis methods
Evaluate and interpret experimental results
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|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
|
| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
Application of biochemical analysis methods, evaluation and interpretation of experimental results with theoretical knowledge. |
|
| Course Contents |
: |
Determination of vitamin C in fruit juice, Determination of urea in serum, Isolation of DNA, Determination of Hemoglobin in whole blood, Polarimeter experiment, Determination of molecular weight of proteins with SDS-PAGE electrophoresis, Lowry protein method, Lowry protein method, Immobilization of lipase onto Celite. |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Laboratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Determination of vitamin C in fruit juice |
Biochemistry books, lecture notes |
Experiment |
|
2 |
Determination of urea in serum |
Biochemistry books, lecture notes |
Experiment |
|
3 |
Isolation of DNA |
Biochemistry books, lecture notes |
Experiment |
|
4 |
Determination of Hemoglobin in whole blood |
Biochemistry books, lecture notes |
Experiment |
|
5 |
Polarimeter experiment |
Biochemistry books, lecture notes |
Experiment |
|
6 |
Determination of molecular weight of proteins with SDS-PAGE electrophoresis |
Biochemistry books, lecture notes |
Experiment |
|
7 |
Determination of molecular weight of proteins with SDS-PAGE electrophoresis |
Biochemistry books, lecture notes |
Experiment |
|
8 |
Midterm exam |
Biochemistry books, lecture notes |
Exam |
|
9 |
Lowry protein method |
Biochemistry books, lecture notes |
Experiment |
|
10 |
Determination of lipase by titration |
Biochemistry books, lecture notes |
Experiment |
|
11 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
12 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
13 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
14 |
Make-up experiments |
Biochemistry books, lecture notes |
Experiment |
|
15 |
Make-up experiments |
Biochemistry books, lecture notes |
Experiment |
|
16/17 |
Final exam |
Biochemistry books, lecture notes |
Exam |
|
|
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Tükel, S. Seyhan (2001).General Biochemistry Lab Book, Ç.Ü. Science Faculty,Chemistry Deparment, Adana.
Söylemez, Z., Fadıloğlu, S., 1996. Enzyme purification and Immobilization, Gaziantep University Faculty of Engineering, Gaziantep.
Güner, S., Çolak, A., Küçük, M., Dinçer, B., Sağlam, N., Doğan, N., 2001. Basic biochemistry techniques and applications. Karadeniz Technical Ü. Science Faculty, Trabzon.
|
| |
| Required Course Material(s) | |
|
|
|
Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
50 |
|
Homeworks/Projects/Others |
14 |
50 |
|
Total |
100 |
|
Rate of Semester/Year Assessments to Success |
40 |
| |
|
Final Assessments
|
100 |
|
Rate of Final Assessments to Success
|
60 |
|
Total |
100 |
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Feel comfortable with chemistry knowledge and capable to make relation with practical applicaitons |
5 |
|
2 |
Observe and analyze the developments, directions and needs of industires for sustainability |
4 |
|
3 |
Acquire life long education capability |
4 |
|
4 |
Have capability of reaching for information |
4 |
|
5 |
Acknowledge about total quality and relating the knowledge from different disciplines |
3 |
|
6 |
Have capability of evaluating the national sources for technology development |
3 |
|
7 |
Have capability of transmitting the knowledge and relating different disciplines |
4 |
|
8 |
Gain the ability to achieve new knowledge and technology |
5 |
|
9 |
Learn problem solving methodolygy and creative thinking |
5 |
|
10 |
Have capability of bringing together theory and practical applicaiton |
5 |
|
11 |
Feel comfortable with laboratory studies |
4 |
|
12 |
Follow the developments in chemistry industries |
4 |
|
13 |
Monitor progress in the field of chemistry. |
4 |
|
14 |
Have capability of team work and leadership |
4 |
|
15 |
Acquire property of objective and critical view |
4 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
|
Class Time (Exam weeks are excluded) |
14 |
2 |
28 |
|
Out of Class Study (Preliminary Work, Practice) |
14 |
1 |
14 |
| Assesment Related Works |
|
Homeworks, Projects, Others |
14 |
1 |
14 |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
2 |
2 |
|
Final Exam |
1 |
2 |
2 |
|
Total Workload: | 60 |
| Total Workload / 25 (h): | 2.4 |
| ECTS Credit: | 2 |
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