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| Course Description |
|
| Course Name |
: |
Biochemistry Lab |
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| Course Code |
: |
KM 425 |
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| Course Type |
: |
Compulsory |
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| Level of Course |
: |
First Cycle |
|
| Year of Study |
: |
4 |
|
| Course Semester |
: |
Fall (16 Weeks) |
|
| ECTS |
: |
2 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. SEYHAN TÜKEL
|
|
| Learning Outcomes of the Course |
: |
practise basic biochemical analysis methods and learn about their application areas
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|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
To practise basic biochemical analysis methods and interpret them with theoretical knowledge. |
|
| Course Contents |
: |
Laboratory rules and introduction of laboratory equipments, Carbohydrates and their specific reactions, Glucose determination in blood, The properties of amino acids and their reactions, Analysis of amino acids with TLC, Analysis of amino acids with titration (formol titration), Titration curves of amino acids:isoelectric point determination, General reactions of proteins, Determination of protein in serum, Determination of amylase activity in saliva, Determination of alkaline phosphatase activity in serum, General reactions of lipids, Determination of serum cholesterol. |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Laboratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Laboratory rules and introduction of laboratory equipments |
Biochemistry books, lecture notes |
Experiment |
|
2 |
Carbohydrates and their specific reactions |
Biochemistry books, lecture notes |
Experiment |
|
3 |
Glucose determination in blood |
Biochemistry books, lecture notes |
Experiment |
|
4 |
The properties of amino acids and their reactions |
Biochemistry books, lecture notes |
Experiment |
|
5 |
Analysis of amino acids with TLC |
Biochemistry books, lecture notes |
Experiment |
|
6 |
Analysis of amino acids with titration (formol titration) |
Biochemistry books, lecture notes |
Experiment |
|
7 |
Titration curves of amino acids:isoelectric point determination |
Biochemistry books, lecture notes |
Experiment |
|
8 |
Midterm exam |
Biochemistry books, lecture notes |
Exam |
|
9 |
General reactions of proteins |
Biochemistry books, lecture notes |
Experiment |
|
10 |
Determination of protein in serum |
Biochemistry books, lecture notes |
Experiment |
|
11 |
Determination of amylase activity in saliva |
Biochemistry books, lecture notes |
Experiment |
|
12 |
Determination of alkaline phosphatase activity in serum |
Biochemistry books, lecture notes |
Experiment |
|
13 |
General reactions of lipids |
Biochemistry books, lecture notes |
Experiment |
|
14 |
Determination of serum cholesterol |
Biochemistry books, lecture notes |
Experiment |
|
15 |
Make up experiments |
Biochemistry books, lecture notes |
Experiment |
|
16/17 |
Final exam |
Biochemistry books, lecture notes |
Exam |
|
|
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Söylemez, Z., Fadıloğlu, S., 1996. Enzyme purification and Immobilization, Gaziantep University Faculty of Engineering, Gaziantep.
Güner, S., Çolak, A., Küçük, M., Dinçer, B., Sağlam, N., Doğan, N., 2001. Basic biochemistry techniques and applications. Karadeniz Technical Ü. Science Faculty, Trabzon
Tükel, S. Seyhan (2001).General Biochemistry Lab Book, Ç.Ü. Science Faculty,Chemistry Deparment, Adana.
|
| |
| Required Course Material(s) |
All biochemistry lecture and laboratory books, web sites
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|
|
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Assessment Methods and Assessment Criteria |
|
Semester/Year Assessments |
Number |
Contribution Percentage |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
50 |
|
Homeworks/Projects/Others |
14 |
50 |
|
Total |
100 |
|
Rate of Semester/Year Assessments to Success |
40 |
| |
|
Final Assessments
|
100 |
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Rate of Final Assessments to Success
|
60 |
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Total |
100 |
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|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Feel comfortable with chemistry knowledge and capable to make relation with practical applicaitons |
5 |
|
2 |
Observe and analyze the developments, directions and needs of industires for sustainability |
3 |
|
3 |
Acquire life long education capability |
4 |
|
4 |
Have capability of reaching for information |
4 |
|
5 |
Acknowledge about total quality and relating the knowledge from different disciplines |
5 |
|
6 |
Have capability of evaluating the national sources for technology development |
3 |
|
7 |
Have capability of transmitting the knowledge and relating different disciplines |
4 |
|
8 |
Gain the ability to achieve new knowledge and technology |
5 |
|
9 |
Learn problem solving methodolygy and creative thinking |
5 |
|
10 |
Have capability of bringing together theory and practical applicaiton |
5 |
|
11 |
Feel comfortable with laboratory studies |
5 |
|
12 |
Follow the developments in chemistry industries |
2 |
|
13 |
Monitor progress in the field of chemistry. |
4 |
|
14 |
Have capability of team work and leadership |
4 |
|
15 |
Acquire property of objective and critical view |
4 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
|
Class Time (Exam weeks are excluded) |
14 |
3 |
42 |
|
Out of Class Study (Preliminary Work, Practice) |
14 |
1 |
14 |
| Assesment Related Works |
|
Homeworks, Projects, Others |
14 |
1 |
14 |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
2 |
2 |
|
Final Exam |
1 |
2 |
2 |
|
Total Workload: | 74 |
| Total Workload / 25 (h): | 2.96 |
| ECTS Credit: | 3 |
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