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Course Description Course Name : Molecular Biology Lab. Course Code : BL 363 Course Type : Compulsory Level of Course : First Cycle Year of Study : 3 Course Semester : Fall (16 Weeks) ECTS : 2 Name of Lecturer(s) : Res.Asst. NİHAN ARABACI Prof.Dr. HATİCE KORKMAZ GÜVENMEZ Learning Outcomes of the Course : Have enough experience about basic molecular biology applications. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : BL 363 Molecular Biology Lab. Recommended Optional Programme Components : None Aim(s) of Course : theaching base and application areas of current technologies in the processing of molecular biology development Course Contents : The principles of molecular biology laboratories, equipments, laboratory safety The principles of molecular biology laboratories, equipments, laboratory safety Plasmid elimination in bacteria with EtBr Plasmid elimination in bacteria with EtBr Plasmid DNA isolation from prokaryotic cell Plasmid DNA isolation from prokaryotic cell Plasmid DNA transformation to prokaryotic cells Plasmid DNA transformation to prokaryotic cells Protein electrophoresis methods, proteins separation on SDS-PAGE, DNA amplification with PCR Protein electrophoresis methods, proteins separation on SDS-PAGE, DNA amplification with PCR total protein extraction from eukaryotic and prokaryotic cells total protein extraction from eukaryotic and prokaryotic cells The separation of nucleic acids on agarose gel The separation of nucleic acids on agarose gel Language of Instruction : Turkish Work Place : LABORATORY   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 The principles of molecular biology laboratories, equipments, laboratory safety lecture notes, current developments and related papers oral presentation (projection), practice with students 2 The principles of molecular biology laboratories, equipments, laboratory safety lecture notes, current developments and related papers oral presentation (projection), practice with students 3 Plasmid elimination in bacteria with EtBr lecture notes, current developments and related papers oral presentation (projection), practice with students 4 Plasmid elimination in bacteria with EtBr lecture notes, current developments and related papers oral presentation (projection), practice with students 5 Plasmid DNA isolation from prokaryotic cell lecture notes, current developments and related papers oral presentation (projection), practice with students 6 Plasmid DNA isolation from prokaryotic cell lecture notes, current developments and related papers oral presentation (projection), practice with students 7 The separation of nucleic acids on agarose gel lecture notes, current developments and related papers oral presentation (projection), practice with students 8 mid-term-exam 9 The separation of nucleic acids on agarose gel lecture notes, current developments and related papers oral presentation (projection), practice with students 10 total protein extraction from eukaryotic and prokaryotic cells lecture notes, current developments and related papers oral presentation (projection), practice with students 11 total protein extraction from eukaryotic and prokaryotic cells lecture notes, current developments and related papers oral presentation (projection), practice with students 12 Protein electrophoresis methods, proteins separation on SDS-PAGE, DNA amplification with PCR lecture notes, current developments and related papers oral presentation (projection), practice with students 13 Protein electrophoresis methods, proteins separation on SDS-PAGE, DNA amplification with PCR lecture notes, current developments and related papers oral presentation (projection), practice with students 14 Plasmid DNA transformation to prokaryotic cells lecture notes, current developments and related papers oral presentation (projection), practice with students 15 Plasmid DNA transformation to prokaryotic cells lecture notes, current developments and related papers oral presentation (projection), practice with students 16/17 FINAL EXAM   Required Course Resources Resource Type Resource Name Recommended Course Material(s)     Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 100     Homeworks/Projects/Others 1 0 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Develops his/her professional experiences and knowledges by taking elective courses together with basic subjects of biology 5 2 takes responsibility as an individual or in a group to solve unpredicted problems that would be encountered 5 3 plans and manages the projects as a member of team 4 4 informs an indivudual and institutions about the subjects related to biology, and prepare verbal and written presentation to solve the problems 5 5 communicates effectively with colleagues in the world about biological issues and works 1 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 2 28     Out of Class Study (Preliminary Work, Practice) 14 1 14 Assesment Related Works     Homeworks, Projects, Others 1 1 1     Mid-term Exams (Written, Oral, etc.) 1 3 3     Final Exam 1 5 5 Total Workload: 51 Total Workload / 25 (h): 2.04 ECTS Credit: 2