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| Course Description |
|
| Course Name |
: |
Biochemistry Lab 2 |
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| Course Code |
: |
KM 318 |
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| Course Type |
: |
Compulsory |
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| Level of Course |
: |
First Cycle |
|
| Year of Study |
: |
3 |
|
| Course Semester |
: |
Spring (16 Weeks) |
|
| ECTS |
: |
4 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. SEYHAN TÜKEL
|
|
| Learning Outcomes of the Course |
: |
Learn biochemical analysis methods
Evaluate and interpret experimental results
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|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
|
| Recommended Optional Programme Components |
: |
None |
|
| Aim(s) of Course |
: |
Application of biochemical analysis methods, evaluation and interpretation of experimental results with theoretical knowledge. |
|
| Course Contents |
: |
Determination of vitamin C in fruit juice, Determination of urea in serum, Isolation of DNA, Determination of Hemoglobin in whole blood, Polarimeter experiment, Determination of molecular weight of proteins with SDS-PAGE electrophoresis, Lowry protein method, Lowry protein method, Immobilization of lipase onto Celite. |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Laboratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Determination of vitamin C in fruit juice
|
Biochemistry books, lecture notes |
Experiment |
|
2 |
Determination of urea in serum |
Biochemistry books, lecture notes |
Experiment |
|
3 |
Isolation of DNA |
Biochemistry books, lecture notes |
Experiment |
|
4 |
Determination of Hemoglobin in whole blood |
Biochemistry books, lecture notes |
Experiment |
|
5 |
Polarimeter experiment |
Biochemistry books, lecture notes |
Experiment |
|
6 |
Determination of molecular weight of proteins with SDS-PAGE electrophoresis |
Biochemistry books, lecture notes |
Experiment |
|
7 |
Determination of molecular weight of proteins with SDS-PAGE electrophoresis |
Biochemistry books, lecture notes |
Experiment |
|
8 |
Midterm exam |
Biochemistry books, lecture notes |
Exam |
|
9 |
Lowry protein method
|
Biochemistry books, lecture notes |
Experiment |
|
10 |
Determination of lipase by titration |
Biochemistry books, lecture notes |
Experiment |
|
11 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
12 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
13 |
Immobilization of lipase onto Celite |
Biochemistry books, lecture notes |
Experiment |
|
14 |
Make-up experiments |
Biochemistry books, lecture notes |
Experiment |
|
15 |
Make-up experiments |
Biochemistry books, lecture notes |
Experiment |
|
16/17 |
Final exam |
Biochemistry books, lecture notes |
Exam |
|
|
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Tükel, S. Seyhan (2001).General Biochemisrty Laboratory, Ç.U. Sciences and Letters Faculty, Chemistry Department, Adana.
Söylemez, Z., Fadıloğlu, S., 1996. Enzyme purification and Immobilization, Gaziantep University Faculty of Engineering, Gaziantep.
|
| |
| Required Course Material(s) | |
|
|
|
Assessment Methods and Assessment Criteria |
|
Semester/Year Assessments |
Number |
Contribution Percentage |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
50 |
|
Homeworks/Projects/Others |
14 |
50 |
|
Total |
100 |
|
Rate of Semester/Year Assessments to Success |
40 |
| |
|
Final Assessments
|
100 |
|
Rate of Final Assessments to Success
|
60 |
|
Total |
100 |
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Feel comfortable with chemistry knowledge and capable to make relation with practical applicaitons |
5 |
|
2 |
Observe and analyze the developments, directions and needs of industires for sustainability |
4 |
|
3 |
Acquire life long education capability |
4 |
|
4 |
Have capability of reaching for information |
4 |
|
5 |
Acknowledge about total quality and relating the knowledge from different disciplines |
3 |
|
6 |
Have capability of evaluating the national sources for technology development |
3 |
|
7 |
Have capability of transmitting the knowledge and relating different disciplines |
4 |
|
8 |
Gain the ability to achieve new knowledge and technology |
5 |
|
9 |
Learn problem solving methodolygy and creative thinking |
5 |
|
10 |
Have capability of bringing together theory and practical applicaiton |
5 |
|
11 |
Feel comfortable with laboratory studies |
4 |
|
12 |
Follow the developments in chemistry industries |
4 |
|
13 |
Monitor progress in the field of chemistry. |
4 |
|
14 |
Have capability of team work and leadership |
4 |
|
15 |
Acquire property of objective and critical view |
4 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
|
|
| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
|
Class Time (Exam weeks are excluded) |
14 |
4 |
56 |
|
Out of Class Study (Preliminary Work, Practice) |
14 |
2 |
28 |
| Assesment Related Works |
|
Homeworks, Projects, Others |
14 |
1 |
14 |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
6 |
6 |
|
Final Exam |
1 |
8 |
8 |
|
Total Workload: | 112 |
| Total Workload / 25 (h): | 4.48 |
| ECTS Credit: | 4 |
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