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Course Description Course Name : Molecular technique in Entomology Course Code : BK-596 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. KAMİL KARUT Learning Outcomes of the Course : 1. Knows isolation techniques of insect DNA 2. Learns cloning insect DNA by Polymerase Chain Reaction (PCR) technique 3. Learns using different PCR technique in entomology 4. Learns gel electrophoresis technique 5. Learns about DNA sequencing and use in entomology Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : Providing information about using molecular technique in classical entomology and giving information about recent developments of this subject in entomology Course Contents : DNA isolation of insect, different PCR techniques used in entomology and gel electrophoresis and DNA sequencing and use in entomology Language of Instruction : Turkish Work Place : Classroom, laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Introduction Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Lecture 2 Application of molecular techniques in entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Lecture 3 DNA isolation techniques from insect Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Lecture 4 DNA isolation techniques from insect Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Lecture 5 “Polymerase Chain Reaction” (PCR) and applications in entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 6 “Polymerase Chain Reaction” (PCR) and applications in entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 7 Different PCR techniques used in Entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 8 Different PCR techniques used in Entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 9 Quiz 10 Different PCR techniques used in Entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 11 Different PCR techniques used in Entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 12 Gel electrophoresis Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 13 Gel electrophoresis Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 14 Sequence of insect DNA and assessment in entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 15 Sequence of insect DNA and assessment in entomology Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Lecture 16/17 Final Exam preparation Final Exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Insect Molecular Genetics (Hoy M) Molecular Systematics (Hillis D.M., Moritz C., Mable B.K.) Moleküler biyolojide kullanılan yöntemler (Temizkan G., Arda N.) Moleküler biyoloji (Yıldırım A., Bardakçı F., Karataş M. ve Tanyolaç B.) Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 1 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has knowledge on basic topics about general science, general agricultural engineering and plant production 4 2 Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants 2 3 Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice 4 4 Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods 5 5 Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly 5 6 Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports 5 7 Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 1 10 10     Mid-term Exams (Written, Oral, etc.) 1 15 15     Final Exam 1 30 30 Total Workload: 139 Total Workload / 25 (h): 5.56 ECTS Credit: 6