| Course Description |
|
| Course Name |
: |
Tissue Culture Techniques |
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| Course Code |
: |
TBP354 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
First Cycle |
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| Year of Study |
: |
3 |
|
| Course Semester |
: |
Spring (16 Weeks) |
|
| ECTS |
: |
4 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. HAKAN ÖZKAN
Prof.Dr. RÜŞTÜ HATİPOĞLU
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|
| Learning Outcomes of the Course |
: |
Learns tools and equipment required in the tissue culture laboratory
Learns the principles of sterile working
Learns medium preparation for tissue culture
Learns isolation and culture of plant explant
Learns processes of tissue culture techniques
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|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
To teach tissue culture techniques which can be used in breeding of field crops |
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| Course Contents |
: |
This lesson contains folliwing topics: tissue culture laboratory and its organization, components of the nutrient medium for tissue culture, chemical used, properties of the chemicals used in tissues culture, solution preparation techniques in tissue culture, techniques of sterilization in tissues culture, techniques of medium preparation, techniques of meristem, callus, cell and protoplast cultures, techniques of obtaining haploid planst in vtro, somaclonal variation and somaic hybridization |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Classroom |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Introduction to tissue culture laboratory and its organization. |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
2 |
Components of the nutrient medium for tissue culture, chemical used, properties of the chemicals used in tissues culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
3 |
Solution preparation techniques in tissue culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
4 |
Preparation of stock solutions for mineral elements |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
5 |
Preparation of stock solutions (vitamins, hormone etc) |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
6 |
Techniques of sterilization in tissues culture and homework will be given on this topic |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
7 |
Techniques of medium preparation |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
8 |
Techniques of explant preparation in tissue culture. |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
9 |
Sterilization of plant materials in tissues culture, isolation of explant and its culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
10 |
Midterm examination |
Preparation to mid-term exam |
Written examination. |
|
11 |
Definition, application and uses of embryo culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
12 |
Meristem culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
13 |
Callus ,cell and protoplast culture |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
14 |
Techniques of obtaining haploid plants in vitro |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
15 |
Somaclonal variation and somatic hybridization |
Reading lecture topic from the course book and the articles about the topic |
Explaining the lecture topic by power point presentation and discussion. |
|
16/17 |
Final examination |
Preparation to final examination |
Written examination |
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|
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Bitki Biyoteknolojisi I Doku Kültürü ve Uygulamaları, 2001. M. BABAOĞLU, E. GÜREL, S. ÖZCAN. S. U. Vakfı Yayınları, Konya. pp.1-456
Bitki Biyoteknolojisi, 1999. R. HATİPOĞLU, Ç.Ü. Ziraat Fakültesi Genel Yayın No: 190 Ders Kitapları Yayın No:A-58
Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories, 1984. Eds. E.F. George and P.D. SHERRINGTON, Exegetics Ltd., England
Handbook of Plant Cell Culture, Volume 1. Techniques for Propagation and Breeding, 1983. Eds. D.E. EVANS, W.R. SHARP, P.V. AMMİRATO, Y. YAMADA Macmillan Publishing Co. New York, pp. 1-970
Experiments in Plant Tissue Culture, 1982. J.H. DODDS AND L.W. ROBERTS, Cambridge University Press, UK
Cell Culture and Somatic Cell Genetics of Plants, Volume 1 Laboratory Procedures and Their Applications, 1984. Ed. I.K. Vasil. Academic Press, Inc. New York, pp. 1-825
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| |
| Required Course Material(s) | |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Has knowledge about agricultural engineering as well as agronomy and breeding of field crops. |
4 |
|
2 |
Determines and solves the problems related to agricultural engineering as well as agronomy and breeding of field crops. |
4 |
|
3 |
Graduates gain abilty to synthetize the basic concepts related to the field crops.
|
4 |
|
4 |
Rrecognises problems related to agricultural engineering,makes decisions and takes initiative to solve the problems. |
2 |
|
5 |
Gains knowledge about sustainable agriculture, protection of environment and natural sources, biodiversity and conservation of genetic sources. |
4 |
|
6 |
Gains ability to optimize the plant production by sustainable use of natural resources. |
4 |
|
7 |
Learns basic principles of breeding and biotechnology of field crops. |
5 |
|
8 |
Chooses and uses modern technical equipments for the agricultural engineering applications as well as for the applications in the agronomy and breeding of field crops. |
4 |
|
9 |
Gains ability to establish suitable research experiments for the purpose and the ability to interpret its results by scientific methods. |
3 |
|
10 |
Works both individually and in a team. |
1 |
|
11 |
Internalizes the necessity of lifelong learning. |
1 |
|
12 |
Has an effective and healthy communication in his fıeld and use communication technologies. |
1 |
|
13 |
Improve themselves consistently by determining educational requirements in scientific, cultural and social areas depending on their abilities,besides their career development |
0 |
|
14 |
Shows respect to job ethic. |
2 |
|
15 |
Becomes competent in the legislation and management systems related to agricultural engineering. |
3 |
|
16 |
Becomes proficient in doing, applying, managing and monitoring plans and projects about agricultural engineering |
2 |
|
17 |
Evaluates the learned knowledge by analytical and critical approach. |
3 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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