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Course Description Course Name : Methods for Identifying Genes and Gene Expression in Plants Course Code : BB-614 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. YILDIZAKA KAÇAR Learning Outcomes of the Course : Obtains conceptual and theoretical information about the methods detecting gene expression level in horticulture breeding. Obtains information about the methods that can be followed while doing researches. Learns how to transfer theoretical knowledge and research findings into practice. Obtains conceptual and theoretical information about genes and gene expression. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is to the teach methods for identifying genes and gene expression in plants. Course Contents : The contents of this course include structure of nucleic acids, protein translation and proteins, mechanism of transcription in plants and protein translation, gene-protein (enzyme), gene-RNA conversions, promotors and determination of promotors by PCR and sequencing techniques in plants, gene banks for promoters, RT PCR analysis to determine the presence of mRNA, determination of the amount of gene expression by qRT-PCR analysis and evaluation of results, determination of gene expression by micro-array chips, and the creation of micro-array chips, bioinformatics. Language of Instruction : Turkish Work Place : The classrooms of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Structure of nucleic acids Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 2 Translation and proteins Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 3 Mechanism of transcription and translation in plants Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 4 Gene-protein (enzyme), gene-RNA conversions Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 5 Gene-protein (enzyme), gene-RNA conversions Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 6 Promotors and determination of promotors by PCR and sequencing techniques in plants Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 7 Promotors and determination of promotors by PCR and sequencing techniques in plants Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 8 Mid-Term Exam Preparation for the mid-term exam Written exam 9 Gene banks for promoters Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 10 Gene banks for promoters Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 11 RT PCR analysis to determine the presence of mRNA Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 12 Determination of the amount of gene expression by qRT-PCR analysis and evaluation of results Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 13 Determining of gene expression by microarray chips, and the creation of microarray chips Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 14 Determining of gene expression by microarray chips, and the creation of microarray chips Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 15 Bioinformatics Reading the related lecture notes. Straight narrative technique, power point presentation and discussion 16/17 Final Exam Preparation of the final exam Written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Analysing gene expression, edited by S. Lorkowski and P. Cullen. 2003. Weinheim, Germany: Wiley-VCH. Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 70     Homeworks/Projects/Others 4 30 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 1 2 Understanding the horticulture and the interaction between the related disciplines 2 3 Solving problems by research methods and establishing cause-effect relationship 3 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 2 5 Carrying out a study individually that requires professional knowledge in Horticulture 1 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 2 7 Leading in the situations when solving problems related to horticulture is necessary 1 8 Entering database related to horticulture, utilizing those sources and self-updating. 0 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 1 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 4 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 1 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 3 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 2 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 4 6 24     Mid-term Exams (Written, Oral, etc.) 1 12 12     Final Exam 1 20 20 Total Workload: 140 Total Workload / 25 (h): 5.6 ECTS Credit: 6