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| Course Description |
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| Course Name |
: |
Applications of Plant Biotechnology in Horticulture |
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| Course Code |
: |
BBP451 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
First Cycle |
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| Year of Study |
: |
4 |
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| Course Semester |
: |
Fall (16 Weeks) |
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| ECTS |
: |
3 |
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| Name of Lecturer(s) |
: |
Prof.Dr. SALİH KAFKAS
Prof.Dr. SAADET BÜYÜKALACA
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| Learning Outcomes of the Course |
: |
Know application of tissue and cell culture in horticulture
Know to shorten breeding program periods in horticulture
Know basics of DNA extraction from leave tissues
Know PCR and electrophoresis in horticulture
Know molecular marker techniques in horticulture
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| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
To teach application of biotechnology in horticulture theoretically and practically |
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| Course Contents |
: |
Introduction to biotechnology and biotechnology in horticulture, meristem and shoot tip culture in horticulture, embryo rescue in horticulture, single nodle and tissue culture cutting in horticulture, haploidization with anter culture in horticulture, gynogenesis in horticulture, somatic embryogenesis with organogenesis in horticulture, units in nucleic acid applications, solutions and buffers, DNA isolation from plant tissues, quantification and qualification of isolated DNA, Polymerase chain reaction, marker assisted selection, molecular marker techniques and evaluation of the data |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Classrooms and Molecular Genetic Laboratory of Department of Horticulture, Faculty of Agriculture, University of Cukurova
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
Introduction to biotechnology and biotechnology in horticulture, |
Reading text notes |
Text notes and related book chapters |
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2 |
meristem and shoot tip culture in horticulture, |
Reading text notes |
Text notes and related book chapters |
|
3 |
embryo rescue in horticulture, |
Reading text notes |
Text notes and related book chapters |
|
4 |
single nodle and tissue culture cutting in horticulture, |
Reading text notes |
Text notes and related book chapters |
|
5 |
haploidization with anter culture in horticulture, |
Reading text notes |
Text notes and related book chapters |
|
6 |
gynogenesis in horticulture, |
Reading text notes |
Text notes and related book chapters |
|
7 |
somatic embryogenesis with organogenesis in horticulture |
Reading text notes |
Text notes and related book chapters |
|
8 |
Mid-term exam |
Reading text notes |
An exam from studied subjects untill the week of exam |
|
9 |
units in nucleic acid applications, solutions and buffers, |
Reading text notes |
Text notes and related book chapters |
|
10 |
DNA isolation from plant tissues, |
Reading text notes |
Text notes and related book chapters |
|
11 |
quantification and qualification of isolated DNA, |
Reading text notes |
Text notes and related book chapters |
|
12 |
Polymerase chain reaction, |
Reading text notes |
Text notes and related book chapters |
|
13 |
marker assisted selection, |
Reading text notes |
Text notes and related book chapters |
|
14 |
molecular marker techniques and evaluation of the data |
Reading text notes |
Text notes and related book chapters |
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15 |
Final exam |
Reading text notes |
Final exam from all the studied subjects |
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16/17 |
Final exam |
Reading text notes |
Final exam from all the studied subjects |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Plant Biotechnology (eds Babaoglu, Gurel ve Ozcan)
Molecular cloning: a laboratory manual (J. Sambrook ve D.W.Russel)
Molecular Biology (eds Yıldırım, Bardakci, Karatas and Tanyolac)
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| |
| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
|
Mid-term Exams (Written, Oral, etc.) |
1 |
50 |
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Homeworks/Projects/Others |
2 |
50 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
| |
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Final Assessments
|
100 |
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Rate of Final Assessments to Success
|
60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
To have sufficient theoretical knowledge in basic engineering and agricultural engineering |
4 |
|
2 |
To have sufficient theoretical knowledge in basic horticulture and also fruit, vegetable, vitis and ornamental growing and breeding subjects
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2 |
|
3 |
To have technical kknowledge in subjects related to horticulture |
5 |
|
4 |
Finding the source of environmental, biological, technical and economical problems in horticulture those reduce yield and quality and developing solutions based on researches |
5 |
|
5 |
To define, entitle and grow fruit, vegetable, vineyard and ornamentals |
1 |
|
6 |
Breeding in Horticulture, developing new cultivars and producing propagation materials of new developed varieties |
5 |
|
7 |
Using and applying biotechnology in horticulture |
5 |
|
8 |
Applying methods for preserving genetic resources and environment for sustainable usage in horticulture |
4 |
|
9 |
Irrigation, fertilization, defining pests and diseases, considering and assaying changes after post-harvest storage in horticulture |
2 |
|
10 |
Obatining data in horticulture, researching, considering, recording, consulting and expert ability, project writing and applying |
3 |
|
11 |
Recognizing different roles those enchance the individual skills and undertaking role as a member of the work team and having skills in terms of team work under different conditions |
3 |
|
12 |
Accessing to the relevant information for the purpose, analytical thinking and planning, questioning, having an exploratory and creative vision and using the obtained knowledge for the benefit of society |
3 |
|
13 |
Transfering knowledge to filed working people with different levels, concacting, data presenting. Moreover updating theirselves in terms of science, culture, art and social areas regarding their individual interest and skills. |
3 |
|
14 |
Establishing-managing orchards, greenhouses and vineyards |
1 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
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Class Time (Exam weeks are excluded) |
14 |
3 |
42 |
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Out of Class Study (Preliminary Work, Practice) |
3 |
3 |
9 |
| Assesment Related Works |
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Homeworks, Projects, Others |
2 |
4 |
8 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
6 |
6 |
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Final Exam |
1 |
6 |
6 |
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Total Workload: | 71 |
| Total Workload / 25 (h): | 2.84 |
| ECTS Credit: | 3 |
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