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| Course Description |
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| Course Name |
: |
Molecular Techniques Used in Horticulture Breeding |
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| Course Code |
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BBP352 |
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| Course Type |
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Optional |
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| Level of Course |
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First Cycle |
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| Year of Study |
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3 |
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| Course Semester |
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Spring (16 Weeks) |
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| ECTS |
: |
3 |
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| Name of Lecturer(s) |
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Prof.Dr. YILDIZAKA KAÇAR
Prof.Dr. SALİH KAFKAS
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| Learning Outcomes of the Course |
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Realizing DNA and RNA
Learning molecular markers
Learning PCR
Realizing the methods of electrophoresis
Realizing based on the markers molecular breeding methods in horticulture breeding
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| Mode of Delivery |
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Face-to-Face |
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| Prerequisites and Co-Prerequisites |
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None |
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| Recommended Optional Programme Components |
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None |
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| Aim(s) of Course |
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To teach molecular methods for Horticulture Breeding |
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| Course Contents |
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This lesson cointains DNA and RNA structure, DNA and RNA isolation and methods used for determination the amount of DNA and RNA, the genetic markers and the history, PCR technologies and the use area, electrophoresis systems, molecular marker technologies and the use in the plant breeding, PCR-based molecular markers, determination of the genetic relationships of plants using molecular markers and the use of genetic similarities inplant breeding, the creation of genetic maps, the use of genetic maps in plants breeding, marker assistant selection for plant breeding, molecular methods used in determination gene expression in plant and the use in plant breeding, the use of next generation sequencing technology in plant breeding. |
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| Language of Instruction |
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Turkish |
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| Work Place |
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The classrooms of Department of Horticulture, Faculty of Agriculture, University of Cukurova |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
DNA and RNA structure |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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2 |
DNA and RNA isolation and methods used for determination the amount of DNA and RNA |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
|
3 |
The genetic markers and the history |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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4 |
PCR technologies and the use area |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
|
5 |
Electrophoresis systems |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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6 |
Molecular marker technologies and the use in the plant breeding |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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7 |
PCR-based molecular markers |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
|
8 |
Determination of the genetic relationships of plants using molecular markers and the use of genetic similarities inplant breeding |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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9 |
The creation of genetic maps |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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10 |
Mid-Term Exam. |
Preparation to mid-term exam. |
Written examination |
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11 |
The use of genetic maps in plants breeding |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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12 |
Marker assistant selection for plant breeding |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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13 |
Molecular methods used in determination gene expression in plant and the use in plant breeding |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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14 |
The use of next generation sequencing technology in plant breeding. |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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15 |
General review |
Reading of the papers about the lecture. |
Straight narrative technique, power point presantation and discussion. |
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16/17 |
Final Exam |
Preparation to final exam |
Written examination |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Plant Genotyping The DNA Fingerprinting of Plants (R.J. Henry)
Molecular Markers in Plant Genetics and Biotechnology (D. Vienne)
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| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
100 |
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Homeworks/Projects/Others |
0 |
0 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
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Final Assessments
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100 |
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Rate of Final Assessments to Success
|
60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
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1 |
To have sufficient theoretical knowledge in basic engineering and agricultural engineering |
1 |
|
2 |
To have sufficient theoretical knowledge in basic horticulture and also fruit, vegetable, vitis and ornamental growing and breeding subjects
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5 |
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3 |
To have technical kknowledge in subjects related to horticulture |
4 |
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4 |
Finding the source of environmental, biological, technical and economical problems in horticulture those reduce yield and quality and developing solutions based on researches |
4 |
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5 |
To define, entitle and grow fruit, vegetable, vineyard and ornamentals |
4 |
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6 |
Breeding in Horticulture, developing new cultivars and producing propagation materials of new developed varieties |
4 |
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7 |
Using and applying biotechnology in horticulture |
5 |
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8 |
Applying methods for preserving genetic resources and environment for sustainable usage in horticulture |
5 |
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9 |
Irrigation, fertilization, defining pests and diseases, considering and assaying changes after post-harvest storage in horticulture |
1 |
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10 |
Obatining data in horticulture, researching, considering, recording, consulting and expert ability, project writing and applying |
3 |
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11 |
Recognizing different roles those enchance the individual skills and undertaking role as a member of the work team and having skills in terms of team work under different conditions |
3 |
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12 |
Accessing to the relevant information for the purpose, analytical thinking and planning, questioning, having an exploratory and creative vision and using the obtained knowledge for the benefit of society |
3 |
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13 |
Transfering knowledge to filed working people with different levels, concacting, data presenting. Moreover updating theirselves in terms of science, culture, art and social areas regarding their individual interest and skills. |
2 |
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14 |
Establishing-managing orchards, greenhouses and vineyards |
0 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
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Class Time (Exam weeks are excluded) |
14 |
2 |
28 |
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Out of Class Study (Preliminary Work, Practice) |
13 |
1 |
13 |
| Assesment Related Works |
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Homeworks, Projects, Others |
0 |
0 |
0 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
10 |
10 |
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Final Exam |
1 |
20 |
20 |
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Total Workload: | 71 |
| Total Workload / 25 (h): | 2.84 |
| ECTS Credit: | 3 |
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