| Course Description |
|
| Course Name |
: |
Cytogenetics |
|
| Course Code |
: |
BY-552 |
|
| Course Type |
: |
Optional |
|
| Level of Course |
: |
Second Cycle |
|
| Year of Study |
: |
1 |
|
| Course Semester |
: |
Spring (16 Weeks) |
|
| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. MEHMET TOPAKTAŞ
|
|
| Learning Outcomes of the Course |
: |
1. Teaches the required cytogenetic methods to conduct studies in plants, humans, mice and rats.
2. Conducts cytogenetic investigationş.
|
|
| Mode of Delivery |
: |
Face-to-Face |
|
| Prerequisites and Co-Prerequisites |
: |
None |
|
| Recommended Optional Programme Components |
: |
None |
|
| Aim(s) of Course |
: |
Teaching cytogenetic techniques to examine plant, human and animal chromosomes. |
|
| Course Contents |
: |
Can be found in lecture course. |
|
| Language of Instruction |
: |
Turkish |
|
| Work Place |
: |
Seminar hall and genetics laboratory. |
|
|
Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Plant karyotypes. |
Board and pencil. |
Theoretical lecture. |
|
2 |
Establishing permanent preparations from the root tips to examine and determine cytogenetic abnormalities in mitosis. |
Rooting the onion (bulb), preparing alcohol material and permanent slides from these roots. |
Feulgen squash method. |
|
3 |
Establishing permanent slides by squash method from anthers to examine meiosis in pollen mother cells. |
Preparing alcohol material from plant buds. |
Staining of pollen mother cells with aceto orsein. |
|
4 |
Establishing permanent slides by squash method from anthers to examine meiosis in pollen mother cells. |
Establishing permanent slides from alcohol material. |
Feulgen squash method. |
|
5 |
Establishing blood cultures from human blood and preparation of permanent slides to examine chromosome aberrations in man. |
Taking peripheral blood from human and the inoculation of blood to chromosome medium in sterile culture tubes. |
Establishing microscope slides using standart preparation techniques after 72 hour incubation. |
|
6 |
Establishing blood cultures from human blood and preparation of permanent slides to examine chromosome aberrations in man. |
Microscopic examination and ready-to-examine permanent slides. |
Analysis of human chromosomes at 1000x magnification. |
|
7 |
Establishing blood cultures from human blood and preparation of permanent slides to examine sister chromatid exchange (SCE). |
Preparation of BrdUrd solution. 72-hr incubation of BrdUrd-added whole blood culture. |
Method of Speit (1984) |
|
8 |
Midterm exam. |
Written examination. |
Evaluation of written examination. |
|
9 |
Examination of slides to analyze SCE. |
Microscopic examination and ready-to-examine permanent slides. |
Counting SCE numbers in permanent slides. |
|
10 |
Various banding methods on human chromosomes. |
Board and pencil. |
Theoretical lecture. |
|
11 |
Various banding methods on human chromosomes. |
Board and pencil. |
Theoretical lecture. |
|
12 |
Establishing preparations to examine the X and Y chromosomes. |
Theoretical lecture. |
Explaining the subject using figures. |
|
13 |
Establishing preparations to examine the X and Y chromosomes. |
Preparing clean slides, spatule and other solutions. |
Establishing preprations from buccal mucosa cells. |
|
14 |
In vivo cytogenetic studies in rat bone marrow cells. |
Theoretical lecture of preparation from rat bone marrow. |
Explaining standart preparation technique for the analysis of chromosomes in bone marrow cells. |
|
15 |
In vivo cytogenetic studies in rat bone marrow cells. |
Microscopic examination and ready-to-examine permanent slides. |
Analysis of human chromosomes at 1000x magnification |
|
16/17 |
Final exam. |
Written examination. |
Evaluation of written examination. |
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Has the advanced hypothetical and applied knowledges in biology field |
5 |
|
2 |
Based on the bachelor´s level of qualification, develops and deepens the knowledge as a specialist in the biology |
5 |
|
3 |
Uses the theoretical and practical knowledge he expertises in biology |
5 |
|
4 |
Integrates and interprets the knowledge from different disciplines by his expertise in biology and generates new information. |
5 |
|
5 |
Solves the problems of biology by using research methods.
|
5 |
|
6 |
Carries out an independent study requires expertise on biology |
5 |
|
7 |
Develops and produces new strategic approaches and solves the unexpected problems in biology area by taking responsibilty |
5 |
|
8 |
Assesses the knowledge he expertises through critical approach and makes leadership solving problems related to biological environments. |
5 |
|
9 |
Systematically transfers the current developments in the field of biology and his/her studies to the person in biology field or other related fields by supporting qualitative and quantitative data |
5 |
|
10 |
Uses at least one foreign language to keep up to date with the field to take part in scientific and social interaction groups to share information at international levels |
0 |
|
11 |
Uses information and communication technologies as required in the biology field |
0 |
|
12 |
In biology, uses statistical software programme to carry out scientific research and also uses information and communication technology in proficiency level of knowledge and skills to critically evaluate |
1 |
|
13 |
Develops the strategy, policy and implementation plans in the issues related to the field of biology and treats the results obtained within standart of quality |
4 |
|
14 |
Uses the knowledge, problem solving, and / or practical skills obtained in the Biology Field in interdisciplinary studies. |
5 |
|
15 |
Oversees social, scientific, cultural and ethical values in order to collect, implement, interpret data in the Biology area |
4 |
|
16 |
Has the ability to formulate and solve the problems of biology in advanced level |
4 |
|
17 |
Has ability to use his advanced knowledge in biology field for thinking systematically and solve problems in multidisciplinary approaches |
5 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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