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Course Description Course Name : Use of Molecular Techniques in Horticultural Plants Course Code : BB-570 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. YILDIZAKA KAÇAR Learning Outcomes of the Course : Obtains conceptual and theoretical information about the molecular methods used in horticulture breeding. Acquires practical skills in molecular techniques used in breeding. Obtains information about the methods which can be followed while doing researches. Learns how to transfer theoretical knowledge and research findings into practice. Obtains information about the problems and solutions encountered in molecular genetic lab. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is to introduce a detailed description of the molecular methods used in horticulture breeding and practical applications. Course Contents : The contents of this course include introduction to molecular genetic lab, solution preparation methods, DNA isolation in plants, determination of DNA quantity and quality, PCR, preparation of agarose and polyacrilamide gel, the use of biochemical markers in breeding, hybridization-based molecular techniques and practices used in horticulture breeding, determination of gen expression (cDNA-AFLP, microarray), quantitative determination of gene expression (Real Time PCR), similarity index, the creation and evaluation of the dendograms. Language of Instruction : Turkish Work Place : The classrooms and plant biotechnology lab. of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Introduction to molecular genetic lab, solutions preparation methods Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 2 DNA isolation in plants Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 3 Determination of DNA quantity and quality Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 4 Polymerase chain reaction (PCR) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 5 Preparation of agarose and polyacrilamide gel Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 6 The use of biochemical markers in breeding Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 7 Mid-Term Exam Preparation to the exam Written exam 8 Hybridization-based molecular techniques and practices used in Horticulture breeding (RFLP) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 9 PCR-based molecular breeding techniques and practices used in Horticulture (RAPD, ISSR) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 10 PCR-based molecular breeding techniques and practices used in Horticulture (SSR, SRAP) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 11 PCR-based molecular breeding techniques and practices used in Horticulture (AFLP, CAPs) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 12 Determination of gen expression (cDNA-AFLP, microarray) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 13 Quantitative determination of gene expression (Real Time PCR) Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 14 Similarity index, the creation and evaluation of the dendograms Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 15 General review Reading course-related information from different sources Straight narrative technique, power point presantation, discussion and laboratuar activities. 16/17 General review Reading course-related information from different sources Düz anlatım, gösterim, tartışma ve laboratuvar uygulaması   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Molecular Markers in Plants Robert J. Henry Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 80     Homeworks/Projects/Others 2 20 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 2 2 Understanding the horticulture and the interaction between the related disciplines 2 3 Solving problems by research methods and establishing cause-effect relationship 4 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 2 5 Carrying out a study individually that requires professional knowledge in Horticulture 2 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 2 7 Leading in the situations when solving problems related to horticulture is necessary 2 8 Entering database related to horticulture, utilizing those sources and self-updating. 2 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 3 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 4 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 1 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 2 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 4 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 5 10 50 Assesment Related Works     Homeworks, Projects, Others 2 8 16     Mid-term Exams (Written, Oral, etc.) 1 8 8     Final Exam 1 8 8 Total Workload: 138 Total Workload / 25 (h): 5.52 ECTS Credit: 6