| Course Description |
|
| Course Name |
: |
Molecular Genetic Applications |
|
| Course Code |
: |
BB-568 |
|
| Course Type |
: |
Optional |
|
| Level of Course |
: |
Second Cycle |
|
| Year of Study |
: |
1 |
|
| Course Semester |
: |
Spring (16 Weeks) |
|
| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. SALİH KAFKAS
|
|
| Learning Outcomes of the Course |
: |
Learns how to use the molecular genetic lab. and its rules.
Learns how to prepare solutions, chemicals and buffers in molecular studies.
Learns about the theoretical and practical research methods in molecular genetic.
Gains practical skills on how to transfer research results obtained in molecular genetic to the practical applications.
Learns the about elecrophoresis systems and PCR technology in detail.
|
|
| Mode of Delivery |
: |
Face-to-Face |
|
| Prerequisites and Co-Prerequisites |
: |
None |
|
| Recommended Optional Programme Components |
: |
None |
|
| Aim(s) of Course |
: |
The aim of this course is to help students gain practical skills on molecular techniques. |
|
| Course Contents |
: |
The contents of this course include introduction and use of molecular genetic lab. , security in the lab, units, calculations, chemical substances, buffers, preparation of stock buffers and their uses, DNA extraction in plants, determination of DNA amount and quality, Gel electrophoresis systems, determination of amount of DNA in agarose gels, extraction of DNA band from agarose gel, cloning of extracted DNA from the gel, sequencing of cloned DNA band, Library types, genomic, chromosomal and DNA libraries, PCR and applications, problems and solutions in PCR reactions |
|
| Language of Instruction |
: |
Turkish |
|
| Work Place |
: |
Classrooms and Molecular Genetic Laboratory of Department of Horticulture, Faculty of Agriculture, University of Cukurova
|
|
|
Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Introduction and use of molecular genetic lab. security in the lab. |
Reading related course notes |
Course notes and related book chapters |
|
2 |
Units and calculations in molecular genetic lab |
Reading related course notes |
Course notes and related book chapters |
|
3 |
Chemical substances, buffers, preparation of stock buffers and their uses in molecular genetic lab |
Reading related course notes |
Course notes and related book chapters |
|
4 |
DNA extraction in plants, |
Reading related course notes |
Course notes and related book chapters |
|
5 |
DNA extraction in plants, |
Reading related course notes |
Course notes and related book chapters |
|
6 |
Determination of DNA amount and quality, |
Reading related course notes |
Course notes and related book chapters |
|
7 |
Gel electrophoresis systems |
Reading related course notes |
Course notes and related book chapters |
|
8 |
Mid-term exam |
Reading related course notes |
An exam from studied subjects until the exam week
|
|
9 |
Extraction and cloning of DNA band from agarose gel, |
Reading related course notes |
Course notes and related book chapters |
|
10 |
Sequencing of cloned DNA band |
Reading related course notes |
Course notes and related book chapters |
|
11 |
Genomic, chromosomal and DNA libraries |
Reading related course notes |
Course notes and related book chapters |
|
12 |
Polymerase chain reaction |
Reading related course notes |
Coures notes and related book chapters |
|
13 |
Polymerase chain reaction applications |
Reading related course notes |
Course notes and related book chapters |
|
14 |
Problems and solutions in PCR reactions |
Reading related course notes |
Course notes and related book chapters |
|
15 |
Problems and solutions in PCR reactions |
Reading related course notes |
Course notes and related book chapters |
|
16/17 |
Final exam |
Reading related course notes |
Final exam from all the studied subjects
|
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications |
4 |
|
2 |
Understanding the horticulture and the interaction between the related disciplines |
2 |
|
3 |
Solving problems by research methods and establishing cause-effect relationship |
1 |
|
4 |
Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. |
3 |
|
5 |
Carrying out a study individually that requires professional knowledge in Horticulture |
2 |
|
6 |
Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results |
2 |
|
7 |
Leading in the situations when solving problems related to horticulture is necessary |
3 |
|
8 |
Entering database related to horticulture, utilizing those sources and self-updating. |
1 |
|
9 |
Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process |
1 |
|
10 |
Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments |
3 |
|
11 |
Using software related to horticulture sufficiently and using information and communication technologies in advanced level |
3 |
|
12 |
Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values |
4 |
|
13 |
Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating |
1 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
|
|