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Course Description Course Name : Physiology of the in vitro Grown Plants Course Code : BB-518 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. SAADET BÜYÜKALACA Learning Outcomes of the Course : The student repeats the basic physiology information Students learn the physiological changes that occur in plants grown in tissue culture Understand the basic needs of plants grown in tissue culture. Students evaluate the status of plants grown in tissue culture and decide what to do Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is to express physiological events occurring in plants developed in vitro Course Contents : This course concentrates on the physiological aspects of tissue culture like nutritional requirements of explants, plant growth regulators required for differentiation. Also, problems like vitrification, tissue browning, shoot tip necrosis and restricted gas exhance are examined. Language of Instruction : English Work Place : Under graduate classroom of horticulture department   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Some aspects of plant physiology Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 2 Comparation of in vitro, ex vitro and in vivo grown plants Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 3 Nutrient needs of plants growing in vitro: macro elements Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 4 Nutrient needs of plants growing in vitro: micro elements Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 5 Loss of water the plants in vitro conditions and changes in the stoma Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 6 Carbon compounds needs of in vitro grown plants Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 7 Mid-term exam Preparation to mid-term exam Written examination 8 Effect of plant growth regulators In vitro: auxins and cytokinins Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 9 Effect of plant growth regulators In vitro: abscisic acid, ethelene and others Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 10 Gas exchange in vitro Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 11 Effect of light on in vitro Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 12 Acclimatization of micropropagated plants to ex vitro conditions Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 13 Physiological and developmental problems encountered by in vitro cultured plants:Shoot-tip necrosis Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 14 Physiological and developmental problems encountered by in vitro cultured plants: tissue browning Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 15 Epigenetic changes and somaclonal variation Preparation from recommended readings and the lecture notes Straight narrative technique, powerpoint presentation and discussion 16/17 Final exam Preparation to final exam Written examination   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Power point presentation prepared by lecturer  Vitrification: morphological and physiological disorders of in vitro plants M. Ziv  In Vitro culture of higher plants. R.L.M. Pierik  Plant Tissue Culture Procedure - Background Edwin F. George, Michael A. Hall, Geert-Jan De Klerk Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 50     Homeworks/Projects/Others 4 50 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 5 2 Understanding the horticulture and the interaction between the related disciplines 4 3 Solving problems by research methods and establishing cause-effect relationship 4 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 4 5 Carrying out a study individually that requires professional knowledge in Horticulture 2 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 4 7 Leading in the situations when solving problems related to horticulture is necessary 2 8 Entering database related to horticulture, utilizing those sources and self-updating. 4 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 3 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 3 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 1 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 3 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 3 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 4 56 Assesment Related Works     Homeworks, Projects, Others 4 6 24     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 10 10 Total Workload: 142 Total Workload / 25 (h): 5.68 ECTS Credit: 6