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Course Description Course Name : Antigen and Antibody reactions Course Code : TİM-507 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 4 Name of Lecturer(s) : Prof.Dr. AKGÜN YAMAN Learning Outcomes of the Course : Explains the polyclonal antibody and how they are produced. Describes the term of monoclonal antibody. Explains the purification of antigen and antibody. Clarifies the terms of affinity and avidity. Explains how to detect antigens using EIA, and fluorescence antibody tests. Defines the general characteristics of antigen-antibody reactions. Describes the terms of prezone,optimal zone and post zone and also explains their importance. Makes a list of qualitative measurements. Makes a list of quantitative measurements. Defines making dilutions and their importance. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : TİM-503 Antigens TİM-504 Antibodies Recommended Optional Programme Components : None Aim(s) of Course : The aim of this lesson is to explain how to do immunological tests and use them in diagnosis of diseases. Course Contents : Characteristics of antibodies and types of antibodies,using antibodies in diagnosis of diseases, terms of affinty, avidity and dilutions and qualitative and quantitative analyzes are taught through theorical lessons and exercises. Language of Instruction : Turkish Work Place : C.U Faculty of Medicine Department of Medical Microbiology meeting room   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Polyclonal antibodies Reading resources and lecture notes on the subject Verbal lecture 2 Monoclonal antibodies Reading resources and lecture notes on the subject Verbal lecture 3 Purification of antigen and antibody Reading resources and lecture notes on the subject Verbal lecture,presentation 4 Affinity Reading resources and lecture notes on the subject Verbal lecture 5 Avidity Reading resources and lecture notes on the subject Verbal lecture 6 Indirect tests Reading resources and lecture notes on the subject, article research Verbal lecture,presentation, discussion 7 Direct tests Reading resources and lecture notes on the subject, article research Verbal lecture,presentation, discussion 8 Midterm exam Exam preperation Written exam 9 Characteristics of antigen-antibody reactions Reading resources and lecture notes on the subject Verbal lecture 10 The term of ´prezone´ Reading resources and lecture notes on the subject Verbal lecture,presentation 11 The term of ´optimal zone´ Reading resources and lecture notes on the subject Verbal lecture,presentation 12 The term of ´post zone´ Reading resources and lecture notes on the subject Verbal lecture,presentation 13 Qualitative analyzes Reading resources and lecture notes on the subject Verbal lecture,presentation 14 Quantitative analyzes Reading resources and lecture notes on the subject Verbal lecture,presentation 15 Dilutions and their importance Reading resources and lecture notes on the subject Verbal lecture,presentation 16/17 Final exam Exam preperation Written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Delves PJ, Martin SJ, Burton DR, Roitt IM, Translation editor: Ilıman MN, Assitant editorı: Yıldız M, Temel İmmünoloji. Atlas Booktrade. 2008  Male D, Brostoff J, Roth DB, Roitt I, Immunology. seventh edition Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 2 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Identifies morphological and physiological characteristics of microorganisms 1 2 Identificates genomic structures of microorganisms 1 3 Explains microorganisms virulance factors and infection mechanisms. 3 4 Defines the mechanisms of immune response and defense against microorganisms 4 5 Lists the antimicrobial agents. 1 6 Defines the mechanisms of resistance development against antimicrobial agents. 1 7 Lists the microscopy, culture, serological and molecular diagnostic principles used in the laboratory diagnosis of infectious microorganisms. 3 8 Lists microbiological tests for identification of microorganisms 1 9 Implements and applies the methods used in the period from receipt of the clinical specimens up to identification of the causative agent . 4 10 Prepares a suitable media, applicates the staining methods, biochemical tests and molecular diagnostic methods to identify microorganisms from clinical samples. 1 11 Applies the biochemical tests and basic microbiological staining methods which are necessary for microbiological identification . 3 12 Determines the microorganisms by using basic molecular diagnostic techniques. 0 13 Works individually or as a team member related to the field. 5 14 Carries out the missions given within the scope of research projects. 5 15 Develops a positive attitude and behaviour towards lifelong learning. 5 16 Evaluates own knowledge and new-learned knowledge in a critical view after graduating. 5 17 Communicates effectively. 5 18 Transfers their own ideas and developments in the field, and offers solutions visually and verbally supporting with qualitative and quantitative data. 4 19 Applies ethical principles during study, evaluation and result sharing. 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 2 3 6     Mid-term Exams (Written, Oral, etc.) 1 5 5     Final Exam 1 10 10 Total Workload: 105 Total Workload / 25 (h): 4.2 ECTS Credit: 4