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Course Description Course Name : Diagnosis of Viral Infections in Laboratory Course Code : VİR-503 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 4 Name of Lecturer(s) : Prof.Dr. FÜGEN YARKIN Learning Outcomes of the Course : Defines the collection of clinical specimens for diagnosis of viruses. Describes the direct and indirect diagnosis of viral infections. Lists the culture methods used in the isolation of the virus Describes the methods used for the microscopic diagnosis of viruses. Defines the immunological and serological methods for the diagnosis of viruses. Applies the immunofluorescence assay (IFA) test Applies latex agglutination test. Applies Enzyme-linked immunosorbent assay (ELISA) test. Interprets the results of viral serologic tests used in diagnosis. Describes nucleic acid-based diagnostic methods. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : To provide information about the basic principles, application and evaluation criteria of laboratory tests used in the diagnosis of viral infections is the aim of this course. Course Contents : In this course, the basic characteristics and application forms of the direct diagnosis methods which are based on the determination of virus in clinical specimen as isolation-production (culture), detection of viral antigens (serological), viral nucleic acid (molecular), virus particle (microscopic) and viral inclusion body (histological) and indirect methods based on specific immune response to the virus and also new diagnostic methods are taught through lectures and practices. Language of Instruction : Turkish Work Place : C.U Faculty of Medicine Department of Medical Microbiology convention hall and laboratories. C.U Faculty of Medicine Department of Medical Virology laboratories.   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Clinical Sample selection and collection methods Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 2 Direct and indirect diagnosis of viral infections Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 3 Isolation and detection of virus in cell culture Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 4 Detection of viruses by electron microscopy Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 5 Cytology, Histology, Inclusion bodies, Tzanck test Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 6 Neutralization test Reading resources and lecture notes about the subject Verbal lecture, Demonstration, 7 Hemagglutination inhibition test, Haemadsorption inhibition test Reading resources and lecture notes about the subject, Laboratory preparation Verbal lecture, Demonstration, Laboratory 8 Midterm Exam Exam preperation written examination 9 Agglutination test, Latex agglutination test Reading resources and lecture notes about the subject, Laboratory preparation Verbal lecture, Demonstration, Laboratory 10 Complement fixation test Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Laboratory 11 Enzyme-linked immunosorbent assay (ELISA) Reading resources and lecture notes about the subject, Laboratory preparation Verbal lecture, Demonstration, Laboratory 12 Immunofluorescence assay (IFA) Reading resources and lecture notes about the subject, Laboratory preparation Verbal lecture, Demonstration, Laboratory 13 Western blot (WB), Immunperoksidase test and immune elektrophoresis, IgG Avidity test Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Discussion 14 Interpretation of serological test results Reading resources and lecture notes about the subject, Preparation of sample questions Discussion 15 Detection of viral nucleic acids Reading resources and lecture notes about the subject Verbal lecture, Demonstration, Laboratory 16/17 Final exam Exam preperation Written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Genel Viroloji, Şemsettin ustaçelebi, Pelikan yayınları, 2008  Moleküler klinik ve tanısal viroloji: Prof. Dr. Şemsettin USTAÇELEBİ, Güneş Tıp Kitabevi 2004 Required Course Material(s)  Cellular and Molecular Immunology, 6th Edition Authors: Abul K. Abbas, Andrew H. Lichtman, & Shiv Pillai, 2007   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 2 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Identifies morphological and physiological characteristics of microorganisms 4 2 Identificates genomic structures of microorganisms 0 3 Explains microorganisms virulance factors and infection mechanisms. 0 4 Defines the mechanisms of immune response and defense against microorganisms 0 5 Lists the antimicrobial agents. 0 6 Defines the mechanisms of resistance development against antimicrobial agents. 0 7 Lists the microscopy, culture, serological and molecular diagnostic principles used in the laboratory diagnosis of infectious microorganisms. 5 8 Lists microbiological tests for identification of microorganisms 5 9 Implements and applies the methods used in the period from receipt of the clinical specimens up to identification of the causative agent . 5 10 Prepares a suitable media, applicates the staining methods, biochemical tests and molecular diagnostic methods to identify microorganisms from clinical samples. 5 11 Applies the biochemical tests and basic microbiological staining methods which are necessary for microbiological identification . 5 12 Determines the microorganisms by using basic molecular diagnostic techniques. 0 13 Works individually or as a team member related to the field. 0 14 Carries out the missions given within the scope of research projects. 0 15 Develops a positive attitude and behaviour towards lifelong learning. 0 16 Evaluates own knowledge and new-learned knowledge in a critical view after graduating. 0 17 Communicates effectively. 0 18 Transfers their own ideas and developments in the field, and offers solutions visually and verbally supporting with qualitative and quantitative data. 0 19 Applies ethical principles during study, evaluation and result sharing. 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 2 3 6     Mid-term Exams (Written, Oral, etc.) 1 5 5     Final Exam 1 10 10 Total Workload: 105 Total Workload / 25 (h): 4.2 ECTS Credit: 4