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| Course Description |
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| Course Name |
: |
Cell-Culture |
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| Course Code |
: |
VİR-502 |
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| Course Type |
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Optional |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
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| Course Semester |
: |
Fall and Spring (16 Weeks) |
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| ECTS |
: |
4 |
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| Name of Lecturer(s) |
: |
Prof.Dr. FÜGEN YARKIN
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| Learning Outcomes of the Course |
: |
Describes the isolation of virus in cell culture.
Defines the proper collection procedure of clinical specimens.
Lists the viral transport media,
Describes techniques for viral isolation.
Lists the tools and materials used in the preparation of cell culture.
Defines the SHELL VIAL technique.
Lists the types of cell culture.
Applies the primary cell culture.
Shows the cell cultures passage
Lists the cytopathic effects of viruses in cells
Defines the tests for the detection of viral antigen and genome
Describes the symptoms of virus reproduction in embryonated eggs.
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| Mode of Delivery |
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Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
Understanding the use of cell culture for the isolation of viruses, learning basic knowledge and skills for the methods of cell culture preparation and interpretation of the results. |
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| Course Contents |
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In this course, the preparation and passage of cell cultures (primary, diploid, continuous cell lines), cryopreservation and thawing of the cells, cytopathic effects of viruses in cells, diagnosis of viruses in the cell culture, tests for the detection of viral antigen and genome, symptoms of virus reproduction in embryonated eggs and shell vial technique are taught through theoretical lectures and practices.
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| Language of Instruction |
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Turkish |
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| Work Place |
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C.U Faculty of Medicine Department of Medical Microbiology convention hall and laboratories. C.U Faculty of Medicine Department of Medical Virology laboratories. |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
The importance of virus isolation in cell culture |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
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2 |
Collecting the clinical samples appropriately |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
|
3 |
Viral transport media |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
|
4 |
Viral isolation techniques, cell culture, embryonated chicken eggs |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
|
5 |
Preparation of cell culture, the tubes, plates and bottles used in the cell production, media used in the cell culture production. |
Laboratory Preparation |
Laboratory |
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6 |
Shell vial technique |
Laboratory Preparation |
Laboratory |
|
7 |
Types of cell cultures (primary, diploid, continuous cell lines) |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
|
8 |
Midterm Exam |
Exam Preparation |
Written Exam |
|
9 |
Preparation of primary cell culture |
Laboratory Preparation |
Laboratory |
|
10 |
Passage of cell cultures |
Laboratory Preparation |
Laboratory |
|
11 |
Cryopreservation of cells
Dissolution of the cells |
Laboratory Preparation |
Laboratory |
|
12 |
Cytopathic effects of viruses in cell culture (pyknosis, syncytia etc.)
İnterferens, haemadsorption, hemagglutination |
Laboratory Preparation |
Laboratory |
|
13 |
Viral plaque formation,
Plaque assay
Counting viruses |
Laboratory Preparation |
Laboratory |
|
14 |
Diagnosis of viruses in cell culture,
Neutralization test |
Laboratory Preparation |
Laboratory |
|
15 |
Tests for the detection of viral antigen and genome.
Symptoms of virus reproduction in embryonated eggs |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
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16/17 |
Exam |
Exam Preparation |
Written Exam |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Genel Viroloji, Şemsettin ustaçelebi, Pelikan yayınları, 2008
Moleküler klinik ve tanısal viroloji: Prof. Dr. Şemsettin USTAÇELEBİ, Güneş Tıp Kitabevi 2004
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| |
| Required Course Material(s) |
Cellular and Molecular Immunology, 6th Edition
Authors: Abul K. Abbas, Andrew H. Lichtman, & Shiv Pillai, 2007
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
60 |
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Homeworks/Projects/Others |
2 |
40 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
| |
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Final Assessments
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100 |
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Rate of Final Assessments to Success
|
60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
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1 |
Identifies morphological and physiological characteristics of microorganisms |
4 |
|
2 |
Identificates genomic structures of microorganisms |
0 |
|
3 |
Explains microorganisms virulance factors and infection mechanisms. |
0 |
|
4 |
Defines the mechanisms of immune response and defense against microorganisms |
0 |
|
5 |
Lists the antimicrobial agents. |
0 |
|
6 |
Defines the mechanisms of resistance development against antimicrobial agents. |
0 |
|
7 |
Lists the microscopy, culture, serological and molecular diagnostic principles used in the laboratory diagnosis of infectious microorganisms. |
4 |
|
8 |
Lists microbiological tests for identification of microorganisms |
4 |
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9 |
Implements and applies the methods used in the period from receipt of the clinical specimens up to identification of the causative agent . |
4 |
|
10 |
Prepares a suitable media, applicates the staining methods, biochemical tests and molecular diagnostic methods to identify microorganisms from clinical samples. |
5 |
|
11 |
Applies the biochemical tests and basic microbiological staining methods which are necessary for microbiological identification . |
4 |
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12 |
Determines the microorganisms by using basic molecular diagnostic techniques. |
0 |
|
13 |
Works individually or as a team member related to the field. |
0 |
|
14 |
Carries out the missions given within the scope of research projects. |
0 |
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15 |
Develops a positive attitude and behaviour towards lifelong learning. |
0 |
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16 |
Evaluates own knowledge and new-learned knowledge in a critical view after graduating. |
0 |
|
17 |
Communicates effectively. |
0 |
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18 |
Transfers their own ideas and developments in the field, and offers solutions visually and verbally supporting with qualitative and quantitative data. |
0 |
|
19 |
Applies ethical principles during study, evaluation and result sharing. |
0 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
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Class Time (Exam weeks are excluded) |
14 |
4 |
56 |
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Out of Class Study (Preliminary Work, Practice) |
14 |
2 |
28 |
| Assesment Related Works |
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Homeworks, Projects, Others |
2 |
3 |
6 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
5 |
5 |
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Final Exam |
1 |
10 |
10 |
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Total Workload: | 105 |
| Total Workload / 25 (h): | 4.2 |
| ECTS Credit: | 4 |
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