| Course Description |
|
| Course Name |
: |
Diagnostic Microbiology |
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| Course Code |
: |
KMB-502 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
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| Course Semester |
: |
Fall and Spring (16 Weeks) |
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| ECTS |
: |
4 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. FATİH KÖKSAL
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|
| Learning Outcomes of the Course |
: |
Defines the system of a microbiology laboratory.
Explains the calculations of chemical substances used in diagnosis.
Defines sterilization, disinfection and antisepsis methods
Summarizes acceptance of clinical laboratory specimens.
Identifies microorganisms by Gram staining.
Describes the methods of media preparation.
Explains the phenotypic, serotypic and genotypic diagnosis methods.
Applies the identification tests manually.
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|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
Getting information about the tools and methods used in microbiological diagnosis and performing diagnostic applications are aimed in this lesson.
|
|
| Course Contents |
: |
Microbiological sampling, handling, making the appropriate culture media, isolation and identification methods are described. |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
C.U Faculty of Medicine Department of Medical Microbiology meeting room and laboratories. |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
The importance of Microbiology Laboratory |
Reading resources and lecture notes about the subject |
Verbal lecture, discussion |
|
2 |
Regulation and rules of the laboratory |
Reading resources and lecture notes about the subject,
Gathering information about the new regulations from the web
|
Verbal lecture, presentation, discussion |
|
3 |
Molarity, normality calculations and the preparation of TTS |
Reading resources and lecture notes about the subject,
Preparation of sample questions |
Verbal lecture, presentation, discussion |
|
4 |
Dilution methods, serial, parallel, and logarithmic dilution |
Reading resources and lecture notes about the subject,
Preparation of sample questions |
Verbal lecture, presentation, discussion |
|
5 |
Sterilization, disinfection and antisepsis
Autoclave, Pasteur´s hot air oven and Koch’s steamer |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation |
|
6 |
Safety cabinets and classification |
Reading resources and lecture notes about the subject,
Collection of foreknowledge about the cabinets |
Verbal lecture, presentation, group work |
|
7 |
Disinfectants and disinfectant combinations |
Reading resources and lecture notes about the subject,
Collection of new information from the web |
Verbal lecture, presentation, discussion |
|
8 |
Midterm exam |
Exam Preperation |
Written exam |
|
9 |
Sampling, handling and acceptance criteria |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation, discussion |
|
10 |
Fresh and stained preparations, staining varieties |
Reading resources and lecture notes about the subject,
Laboratory preparation |
Verbal lecture, presentation, discussion |
|
11 |
Gram staining and special stains |
Reading resources and lecture notes about the subject,
Laboratory preparation |
Laboratory |
|
12 |
Media, the importance and types of additives |
Reading resources and lecture notes about the subject,
Laboratory preparation |
Laboratory |
|
13 |
Inoculation techniques and analysis of the colonies |
Reading resources and lecture notes about the subject,
Laboratory preparation
|
Laboratory |
|
14 |
Phenotypic identification and automated systems |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation |
|
15 |
Serotyping and Genotyping |
Reading resources and lecture notes about the subject |
Verbal lecture, presentation |
|
16/17 |
Final Exam |
Exam Preperation |
Written exam |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Identifies morphological and physiological characteristics of microorganisms |
5 |
|
2 |
Identificates genomic structures of microorganisms |
2 |
|
3 |
Explains microorganisms virulance factors and infection mechanisms. |
2 |
|
4 |
Defines the mechanisms of immune response and defense against microorganisms |
2 |
|
5 |
Lists the antimicrobial agents. |
0 |
|
6 |
Defines the mechanisms of resistance development against antimicrobial agents. |
0 |
|
7 |
Lists the microscopy, culture, serological and molecular diagnostic principles used in the laboratory diagnosis of infectious microorganisms. |
4 |
|
8 |
Lists microbiological tests for identification of microorganisms |
5 |
|
9 |
Implements and applies the methods used in the period from receipt of the clinical specimens up to identification of the causative agent . |
5 |
|
10 |
Prepares a suitable media, applicates the staining methods, biochemical tests and molecular diagnostic methods to identify microorganisms from clinical samples. |
4 |
|
11 |
Applies the biochemical tests and basic microbiological staining methods which are necessary for microbiological identification . |
5 |
|
12 |
Determines the microorganisms by using basic molecular diagnostic techniques. |
3 |
|
13 |
Works individually or as a team member related to the field. |
0 |
|
14 |
Carries out the missions given within the scope of research projects. |
0 |
|
15 |
Develops a positive attitude and behaviour towards lifelong learning. |
0 |
|
16 |
Evaluates own knowledge and new-learned knowledge in a critical view after graduating. |
0 |
|
17 |
Communicates effectively. |
0 |
|
18 |
Transfers their own ideas and developments in the field, and offers solutions visually and verbally supporting with qualitative and quantitative data. |
0 |
|
19 |
Applies ethical principles during study, evaluation and result sharing. |
0 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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