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Course Description |
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Course Name |
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ELECTROPHORETIC DETERMINATION OF ISOENZYMES |
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Course Code |
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BİK-510 |
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Course Type |
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Optional |
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Level of Course |
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Second Cycle |
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Year of Study |
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1 |
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Course Semester |
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Fall and Spring (16 Weeks) |
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ECTS |
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4 |
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Name of Lecturer(s) |
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Prof.Dr. ABDULLAH TULİ Prof.Dr. LEVENT KAYRIN |
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Learning Outcomes of the Course |
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discribes enzyme and isoenzyme owns knowledge about enzyme kinetics knows the discribtion and the theory of electrophoresis knows the usage and application field of enzyme and isoenzymes by electrophoesis and interprets specific samples of the issue owns knowledge about the other application fields of electrophoresis and gel scanning
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Mode of Delivery |
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Face-to-Face |
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Prerequisites and Co-Prerequisites |
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BİK-501 BASIC BIOCHEMISTRY I
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Recommended Optional Programme Components |
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None |
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Aim(s) of Course |
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the aim of the course is to determine enzymes and isoenzymes by electrophoretic methods and their application fields |
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Course Contents |
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Enzyme and isoenzyme, enzyme kinetics, main definition and theory of electrophoresis, application fields of enzyme and isoenzymes by using electrophoresis and specific samples of this issue |
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Language of Instruction |
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Turkish |
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Work Place |
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Classroom |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
Enzyme and isoenzyme |
listening and signification, preparing slides, interpreting the cases, critical thinking, improving questions |
lecturing |
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2 |
Enzyme and isoenzyme |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
lecturing |
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3 |
Enzyme and isoenzyme |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive, assignment, discussion |
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4 |
Enzyme kinetics |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive, laboratory, assignment |
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5 |
Enzyme kinetics |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive |
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6 |
Enzyme kinetics |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive, homework, discussion |
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7 |
General Definition of Electrophresis |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive |
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8 |
General Definition of Electrophresis |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive |
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9 |
Mid-term Examination |
study about the previous lessons |
classic |
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10 |
Sample Studies for Enzymes (Alkaline Phosphatase) |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive and application with case discussion |
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11 |
Sample Studies for Enzymes (Lactate Dehydrogenase) |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive and application with case discussion |
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12 |
Sample Studies for Enzymes (G6PD) |
listening and signification, preparing, slides, interpreting the cases, thinking judgemental, improving questions |
interactive and application with case discussion |
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13 |
Sample Studies for Enzymes (Pyruvate Kinase) |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive and application with case discussion |
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14 |
Sample Studies for Enzymes (Creatine Kinase) |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive and application with case discussion |
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15 |
Gel Scanning |
listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions |
interactive and with laboratory application |
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16/17 |
Final Examination |
study about the previous lessons |
classic |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
Williams BL, Wilson Keith. Principles and Techniques of Practical Biochemistry, Edward Arnold Ltd., 1975.
Segel IH. Enzymes, Chapter 4, in Biochemical Calculations, 2nd Ed., John Wiley & Sons, 1976.
Burtis CA, Achwood ER. Tietz Textbook of Clinical Chemistry, 3rd Ed., Saunders, Philadelphia, 1996.
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| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
50 |
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Homeworks/Projects/Others |
4 |
50 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
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Final Assessments
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100 |
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Rate of Final Assessments to Success
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60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
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1 |
Applies innovations in laboratory processes by doing research. |
1 |
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2 |
Comprehends the interaction of biochemistry with the other scientific fields. |
4 |
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3 |
Knows experimental methods in biochemistry. |
2 |
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4 |
Takes part in in-vitro/in-vivo animal research studies. |
3 |
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5 |
Carries out studies in the field of biochemistry as a member of a team. |
0 |
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6 |
Gains ongoing professional skills. |
4 |
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7 |
Comprehends basic information about the field of biochemistry. |
4 |
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8 |
Interprets the factors and phases that effect the laboratory results. |
1 |
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9 |
Uses laboratory tools. |
2 |
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10 |
Knows the ethical and legal characteristics of laboratory processes, and interpretes economical value of these processes for the country. |
1 |
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11 |
Supports a scientific research. |
2 |
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12 |
Interpretes scientific research results which are carried out. |
2 |
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13 |
Generates a report from the conclusion of the scientific research. |
2 |
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14 |
Knows biochemical sampling rules. |
2 |
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15 |
Studies and makes research in laboratory settings. |
0 |
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16 |
Corporates with the other members of the team in an efficient, sensitive and respectfull way. |
0 |
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17 |
Interpretes and corrolates biochemical data and theoretical concept. |
2 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
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Class Time (Exam weeks are excluded) |
14 |
1 |
14 |
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Out of Class Study (Preliminary Work, Practice) |
14 |
1 |
14 |
| Assesment Related Works |
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Homeworks, Projects, Others |
4 |
10 |
40 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
10 |
10 |
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Final Exam |
1 |
20 |
20 |
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Total Workload: | 98 |
| Total Workload / 25 (h): | 3.92 |
| ECTS Credit: | 4 |
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