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Course Description Course Name : Practical Mycrobiology Course Code : MİK-503 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 4 Name of Lecturer(s) : Asst.Prof.Dr. TOĞRUL NAĞIYEV Learning Outcomes of the Course : Is able to use microbiology laboratory effectively. Explains basic principles of sterilization and disinfection. Explains disinfectants and their uses . Is able to recognize and use sterlization equipments. Implements the methods of staining used in microbiology Prepares the required media and solutions for microbiology. Evaluates cultures. Applies biochemical tests and antibiotic susceptibility testing. Describes the serological tests used in microbiological diagnosis. Explains the molecular tests and PCR types used in microbiology. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aim of this lesson is to provide basic information about the diagnosis of microorganisms by using staining, culture, serology and molecular methods and disinfection and sterilization in microbiology laboratory. Course Contents : In this course, disinfection-sterilization methods and their effective mechanisms, microbiological staining, culture methods, serological and molecular tests, and evoluation of test results are introduced. Language of Instruction : Turkish Work Place : C.U Faculty of Medicine Department of Medical Microbiology meeting room   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Biosids Basic prensiples of disinfection and sterlization Reading resources and lecture notes on the subject Verbal lecture, presentation 2 Disinfectants and their impact mechanisms The disinfections combinations, Sterilization and its types Reading resources and lecture notes on the subject Verbal lecture, presentation 3 The measurement of disinfectant effectiveness New approaches to disinfection and infection controls Alternative disinfection and sterilization methods Reading resources and lecture notes on the subject Verbal lecture 4 Staining methods in microbiology Reading resources and lecture notes on the subject Verbal lecture 5 Media preparation, chemical solution preparation Reading resources and lecture notes on the subject Laboratory preparation Laboratory 6 Culture evaluation and biochemical tests Reading resources and lecture notes on the subject Laboratory preparation Laboratory 7 Antibiotic susceptibility tests Reading resources and lecture notes on the subject Laboratory preparation Laboratory 8 Midterm exam Exam Preparation Written exam 9 Serologic tests Reading resources and lecture notes on the subject,article research Verbal lecture, discussion 10 ELISA, IFA, DFA Reading resources and lecture notes on the subject Verbal lecture 11 Immunblot, indirect hemagglutination Reading resources and lecture notes on the subject Verbal lecture 12 Molecular tests and types of PCR Reading resources and lecture notes on the subject Verbal lecture 13 SDS PAGE Reading resources and lecture notes on the subject Verbal lecture 14 PFGE, AFLP Reading resources and lecture notes on the subject Verbal lecture 15 MIRU VNTR and spoligotyping Reading resources and lecture notes on the subject Laboratory preparation Verbal lecture 16/17 Final exam Exam preperation Written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Infection Control Science, Management and Practice Janet Mc.M. Whurr Pub. 2000.  Grusami M. Bacterial Disinfection and Decontamination Taylor& Francis 2008  Biofilms, Infection, and Antimicrobial Therapy Pace J.L Taylor& Francis 2006 Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 2 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Identifies morphological and physiological characteristics of microorganisms 4 2 Identificates genomic structures of microorganisms 1 3 Explains microorganisms virulance factors and infection mechanisms. 2 4 Defines the mechanisms of immune response and defense against microorganisms 0 5 Lists the antimicrobial agents. 1 6 Defines the mechanisms of resistance development against antimicrobial agents. 1 7 Lists the microscopy, culture, serological and molecular diagnostic principles used in the laboratory diagnosis of infectious microorganisms. 5 8 Lists microbiological tests for identification of microorganisms 2 9 Implements and applies the methods used in the period from receipt of the clinical specimens up to identification of the causative agent . 5 10 Prepares a suitable media, applicates the staining methods, biochemical tests and molecular diagnostic methods to identify microorganisms from clinical samples. 1 11 Applies the biochemical tests and basic microbiological staining methods which are necessary for microbiological identification . 1 12 Determines the microorganisms by using basic molecular diagnostic techniques. 1 13 Works individually or as a team member related to the field. 5 14 Carries out the missions given within the scope of research projects. 5 15 Develops a positive attitude and behaviour towards lifelong learning. 5 16 Evaluates own knowledge and new-learned knowledge in a critical view after graduating. 5 17 Communicates effectively. 5 18 Transfers their own ideas and developments in the field, and offers solutions visually and verbally supporting with qualitative and quantitative data. 5 19 Applies ethical principles during study, evaluation and result sharing. 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 2 5 10     Mid-term Exams (Written, Oral, etc.) 1 5 5     Final Exam 1 10 10 Total Workload: 109 Total Workload / 25 (h): 4.36 ECTS Credit: 4