| Course Description |
|
| Course Name |
: |
Techniques of Electron Microscopy I |
|
| Course Code |
: |
HİS511 |
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| Course Type |
: |
Compulsory |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
|
| Course Semester |
: |
Spring (16 Weeks) |
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| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. UFUK ÖZGÜ METE
Prof.Dr. SAİT POLAT
|
|
| Learning Outcomes of the Course |
: |
Knows the developmental stages of medical cell biology and histology in our university and history.
Recognizes the equipments required in an electron microscopy laboratory.
Explains the principles of techniques used in ultrastructural analysis.
Prepares the solutions, selects an appropriate fixation technique according to the purpose and applies it .
Applies dehydration and infiltration processes.
Applies the embedding process appropriately.
Demonstrates the ability of ultrathin tissue sectioning on the ultramicrotome and placing the specimen onto the grid.
Applies the staining technique.
Shows proficieny to read electron microscopy micrographs.
Proposes a solution to the technical problems which may occur at different stages of preparation and visualisation.
Realizes, assesses and applies the fundamental steps throughout the whole process.
|
|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
Aim of this course is to give basic information about human tissues and cells and to teach and apply the electron microscopic preparation techniques in order to examine human organs and tissues at electron microscobe |
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| Course Contents |
: |
Obtaining the tissues,fixation and fixation solutions,tissue preparation methods, getting tissue sections by using ultramicrotome, staining and electron microscopic examination. |
|
| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Laboratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
Electron microscopic stages in fixation and preparation methods |
Self-study, reading recommended sources |
Demonstration, question-answer, discussion |
|
2 |
Evaluation of cytologic and histologic resolution |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
3 |
Modification of electron microscopic tissue preparation scheme |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
4 |
Important fixatives, introducing main principles of fixatives and its interpretation |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
5 |
Electron microscopy staining techniques |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
6 |
Laboratory practice |
Self-study |
Demonstration, question-answer, discussion |
|
7 |
Evaluation of the results of laboratory studies |
Self-study |
Question-answer, discussion |
|
8 |
Midterm exam |
Self-study, reading recommended sources |
oral exam |
|
9 |
Artifacts occuring during preparation procedure |
Self-study |
Lecture, question-answer, discussion |
|
10 |
Solving problems which occur during preparation procedure |
Self-study |
Lecture, question-answer, discussion |
|
11 |
Visualization methods |
Self-study |
Demonstration, question-answer, discussion |
|
12 |
The importance of diagnostic electron microscopy |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
13 |
Basic knowledge about molecular techniques |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
14 |
Evaluation of electron micrograph method in research projects |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
15 |
Evaluation of electron micrograph method in research projects |
Self-study, reading recommended sources |
Lecture, question-answer, discussion |
|
16/17 |
Final exam |
Self-study, reading recommended sources |
oral exam |
|
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Tell the main histological features of cells and tissues. |
0 |
|
2 |
Corelate the structure and function of cells. |
0 |
|
3 |
Have knowledge of the histologic features of organ systems. |
0 |
|
4 |
Have knowledge of embryo development, developmental disorders and the mechanisms. |
0 |
|
5 |
Define the methods of light and electron microscopic examination and tissue preparation. |
5 |
|
6 |
Have knowledge of general principles about cell culture, histochemistry and immunohistochemistry. |
0 |
|
7 |
Prepare solutions and tissues for light and electron microscopic studies . |
5 |
|
8 |
Apply the basic methods of staining. |
0 |
|
9 |
Distinguish tissue and organ systems in light microscopic level. |
0 |
|
10 |
Perform studies as a team member or indıvidual. |
0 |
|
11 |
Perform tasks which is given in research projects. |
0 |
|
12 |
Develop positive attitudes and behaviors towards lifelong learning. |
0 |
|
13 |
Determine and direct needs and resources of learnings by using resources effectively. |
0 |
|
14 |
Evaluate knowledges and skills of the master degree critically. |
0 |
|
15 |
Communicate effectively. |
0 |
|
16 |
Relay ideas and solution offers about current developments in studies by supporting them with quantitative and qualitative data visually and verbally. |
0 |
|
17 |
Know the importance of the ethical principles in research studies, evaluations and results. |
0 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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