| Course Description |
|
| Course Name |
: |
Introduction to Immunohistochemistry |
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| Course Code |
: |
HİS513 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
Second Cycle |
|
| Year of Study |
: |
1 |
|
| Course Semester |
: |
Fall and Spring (16 Weeks) |
|
| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Asst.Prof.Dr. HÜLYA ÖZGÜR
Prof.Dr. SAİT POLAT
|
|
| Learning Outcomes of the Course |
: |
Defines immunohistochemistry.
Prepares chemical solutions for immunohistochemical techniques.
Selects an appropriate fixation method.
Chooses appropriate dye for the purpose.
Proposes solutions to problems faced when applying the immunohistochemistry method.
Applies and comprehends all steps in immunohistochemistry methods.
Assesses the role of each step.
|
|
| Mode of Delivery |
: |
Face-to-Face |
|
| Prerequisites and Co-Prerequisites |
: |
HİS510 Histochemistry
|
|
| Recommended Optional Programme Components |
: |
None |
|
| Aim(s) of Course |
: |
The aim of this course is to teach the immunohistochemical methods. |
|
| Course Contents |
: |
Immunohistochemical terminology, Antibody and antibody types,Material storage, Fixation,Tissue prosessing,Staining methods, Control tests,Staining problems,Solutions. |
|
| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Laboratory |
|
|
Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
The basic principles of immunocytochemistry |
Self-study, reading recommended soruces |
Presentation |
|
2 |
The basic steps of tissue processes |
Self-study, reading recommended soruces |
Presentation and demonstration |
|
3 |
Immunohistochemical marking methods |
Self-study, reading recommended soruces |
Presentation and demonstration |
|
4 |
Fixation methods and mixtures of fixatives, proper fixation selection, preparation |
Self-study, Review of the literature |
Presentation and demonstration |
|
5 |
Perfusion of experimental animals, taking tissue samples, immersion |
Self-study, Review of the literature |
Demonstration and application |
|
6 |
Avidin-biotin complex method |
Self-study, Review of the literature |
Presentation and demonstration |
|
7 |
DAB preparation, preparation of antibodies |
Self-study,Review of the literature |
Demonstration and application |
|
8 |
Immunohistochemical staining |
Self-study |
Presentation and demonstration |
|
9 |
Preparation of Trypsin, hydrogen peroxide and PBS |
Self-study,Review of the literature |
Demonstration and application |
|
10 |
Homework |
Self-study |
Laboratory practice |
|
11 |
Immunohistochemical staining |
Self-study,Review of the literature |
Demonstration and application |
|
12 |
Immunohistochemical staining |
Self-study,Review of the literature |
Demonstration and application |
|
13 |
Evaluation of preparations |
Self-study |
Presentation and demonstration |
|
14 |
Taking photosand evaluation of photos |
Self-study |
Application |
|
15 |
Homework |
Self-study |
Laboratory practice |
|
16/17 |
Final |
Self-study, reading recommended soruces |
Exam |
|
|
|
Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Kaya, M et al. Genel Histoloji Ders Notları. Ç.Ü. Tıp Fakültesi yayınları. No:10, 2005.
Demir, R. Histolojik boyama teknikleri. Palme yayıncılık. Ankara, 2001.
Luna LG. Manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. Mc Graw Hill book co. 1968.
Naish SJ. Immunohistochemical Staining Methods. Carpinteria, DAKO, 1989.
|
| |
| Required Course Material(s) |
Immunohistochemistry:Pratical Applications in Pathology and Biology, Bristol Wright PSG,1989.
|
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Tell the main histological features of cells and tissues. |
0 |
|
2 |
Corelate the structure and function of cells. |
0 |
|
3 |
Have knowledge of the histologic features of organ systems. |
0 |
|
4 |
Have knowledge of embryo development, developmental disorders and the mechanisms. |
0 |
|
5 |
Define the methods of light and electron microscopic examination and tissue preparation. |
0 |
|
6 |
Have knowledge of general principles about cell culture, histochemistry and immunohistochemistry. |
5 |
|
7 |
Prepare solutions and tissues for light and electron microscopic studies . |
5 |
|
8 |
Apply the basic methods of staining. |
5 |
|
9 |
Distinguish tissue and organ systems in light microscopic level. |
0 |
|
10 |
Perform studies as a team member or indıvidual. |
0 |
|
11 |
Perform tasks which is given in research projects. |
0 |
|
12 |
Develop positive attitudes and behaviors towards lifelong learning. |
0 |
|
13 |
Determine and direct needs and resources of learnings by using resources effectively. |
0 |
|
14 |
Evaluate knowledges and skills of the master degree critically. |
0 |
|
15 |
Communicate effectively. |
0 |
|
16 |
Relay ideas and solution offers about current developments in studies by supporting them with quantitative and qualitative data visually and verbally. |
0 |
|
17 |
Know the importance of the ethical principles in research studies, evaluations and results. |
5 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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