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Course Description Course Name : Laboratory Techniques in Biotechnology Course Code : TB-607 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. RÜŞTÜ HATİPOĞLU Learning Outcomes of the Course : Learn the concept of biotechnology, plant biotechnology and invitro culture Learn how to use the equipment of laboratory Learn steril work, preparation of plant tissues medium, some techniques for in vitro, isolation of DNA and PCR analysis Learn sterile work and safety rules Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : OPTIONAL COURSES Recommended Optional Programme Components : None Aim(s) of Course : To teach the concept of biotechnology, plant biotechnology and in vitro culture, and to give some information about methodologies used in plant biotechnology Course Contents : This course contains the concept of biotechnology, plant biotechnology and in vitro culture, introduction to plant biotechnology laboratory, plant culture medium used in in vitro culture, preparing stock solution for macro and microelements, preparing stock solution for plant hormone and vitamins, sterilization for glass and metal materials, preparing plant culture medium, principles of working in the sterile cabin, sterilization of plant materials, embryo culture, anther culture, DNA isolation and PCR analysis Language of Instruction : Turkish Work Place : Classroom   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Explaining the concept of biotechnology, plant biotechnology and invitro culture Pre reading Using power point, case study and laboratory study 2 Introduction to plant biotechnology laboratory Pre reading Using power point, case study and laboratory study 3 Plant culture medium used in in vitro culture Pre reading Using power point, case study and laboratory study 4 Preparing stock solution for macro and microelements Pre reading Using power point, case study and laboratory study 5 Preparing stock solution for plant hormone and vitamins Pre reading Using powe rpoint, case study and laboratory study 6 Sterilization principles for glass and metal materials Pre reading Using power point, case study and laboratory study 7 Preparing plant culture medium Pre reading Using power point, case study and laboratory study 8 Principles of working the sterile cabin Pre reading Using power point, case study and laboratory study 9 Mid-term exam Exam preparation Written examination 10 Sterilization of plant materials and an assignment will be given on this topic Pre reading Using power point, case study and laboratory study 11 Embryo culture Pre reading Using power point, case study and laboratory study 12 Anther culture Pre reading Using power point, case study and laboratory study 13 DNA isolation Pre reading Using power point, case study and laboratory study 14 PCR analysis Pre reading Using power point, case study and laboratory study 15 Evaluation and interpretation of PCR analysis Pre reading Using power point, case study and laboratory study 16/17 Final exam Exam preparation Written examination   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  HATİPOĞLU, R. , 1999. Bitki Biyoteknolojisi. Çukurova Üniversitesi Ziraat Fakültesi Genel Yayın No: 190, Ders Kitapları Yayın No: A-58. Çukurova Üniversitesi Yayınları No: 197  PIERIK, R.L.M. 1987. In Vitro Culture of Higher Plants. Martinus Righoff Publishers, Dortrecht, Bosto, Lanchester. P. 309  BAJAJ, Y.P.S. 1983. In Vitro Production of Haploids. In: Handbook of Plant Cell Culture Vol. 1: 228-287, P.V. Ammirato, D.A. Evans, W.R. Sharp ve Y. Yamada (eds.), Macmillian Publishing Company, Newyork  McPherson, MJ ve MOLLER, SG. 2006. PCR.Oxford, Uk; BIOS Scientific publishers Ltd  SAMBROOK J. ve RUSSELL DW. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 1 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Graduates become a specialist on the field crops area by improving their skills. 4 2 They comprehend interdisciplinary interaction in his specialization area. 4 3 They can follow the latest developments in field crops area and get access to the knowledge source, gather this knowledge, reach new knowledge and synthesize by evaluating available studies and follow the innovations in his field. 3 4 They can synthesize and interpret interdisciplinary knowledge by using theoretical and practical skills at a specialist level in field crops area. 2 5 They find solutions for problems related to field crops by using research methods and establish cause effect relationships. 4 6 They incorporate their knowledge on his special area by combining their knowledge with those from the other scientific areas and produce new knowledge and also solve the problems by using scientific research methods. 3 7 They determine a problem independently in field crops subject, provide solutions, evaluate the results and implement whenever required. 4 8 They are qualified to solve a problem in their field. 0 9 They are equipped with analytical and critical thinking ability to direct their learning and to conduct advanced studies in their fields independently. 4 10 They transfer current developments and their own studies in the field crops area systematically to the his working group and to different groups from other study fields orally or visually by supporting them with qualitative and quantitive data. 3 11 They study and improve the social relationships and standards leading these relationship by a critical point of view and take action when needed. 0 12 They develop policy, strategy and experimental plans related to field crops area and evaluate obtained results within the framework of quality processes. 0 13 They are sensitive about scientific and ethical values during the collection, interpretion and announcement of data related to their subject.They are also capable of teaching and checking these values. 3 14 They are able to understand and translate an article written in a foreign language. 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 4 56 Assesment Related Works     Homeworks, Projects, Others 1 5 5     Mid-term Exams (Written, Oral, etc.) 1 12 12     Final Exam 1 22 22 Total Workload: 151 Total Workload / 25 (h): 6.04 ECTS Credit: 6