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Course Description Course Name : Medical Bology Labrotory Applications Course Code : BİO526 Course Type : Compulsory Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. HALİL KASAP Prof.Dr. MÜLKİYE KASAP Prof.Dr. OSMAN DEMİRHAN Prof.Dr. HÜSNÜ ÜMİT LÜLEYAP Assoc.Prof.Dr. AYFER PAZARBAŞI Prof.Dr. DAVUT ALPTEKİN Prof.Dr. HÜSNÜ ÜMİT LÜLEYAP Learning Outcomes of the Course : Knows the principles of laboratory practise. Knows how to clean the laboratory. Knows the preliminaries for the practises of the lectures Medical Biology and Genetics, which is given to term-1 students. Knows the materials and devices on cytogenetics and molecular genetics laboratories. Knows material acceptance for routine services, conservation of materials, and studying principles with materials. Has a sense of responsibility for the procedures of routine services. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The purpose of this lecture is to teach students the principles of laboratory practice, cleaning of laboratories, preliminaries for the practises of the lectures Medical Biology and Genetics, which is given to term-1 students and to give an introduction of materials and devices on cytogenetics and molecular-genetics laboratories, material acceptance, conservation of materials, principles of working with materials, and for our routine services, to teach them planning and programming and all the laboratory studies carried out at Medical Biology Department. Course Contents : The content of this lecture is to provide students with the required information about the principles of laboratory practice, cleaning of laboratories, preliminaries for the practises of the lectures Medical Biology and Genetics ,which is given to term-1 students and to give an introduction of materials and devices on cytogenetics and molecular-genetics laboratories, material acceptance for routine services, conservation of materials, principles of working with materials, and to give them a sense of responsibility for the procedures of routine services. Language of Instruction : Turkish Work Place : Laboratory, seminar room   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Principles of laboratory practice Laboratory preparation Visual and practical education in laboratory environment 2 Introduction of the laboratory setting and the devices Device preparation in laboratory environment Visual and practical education in laboratory environment 3 Types of microscopes and the principles involved in using them Preparation of different types of microscopes Visual and practical education in laboratory environment 4 Using the microscope Preparation of different types of microscopes Visual and practical education in laboratory environment 5 Techniques of preparation Preparation of materials such as Lame-Lamellae for the microscope Visual and practical education in laboratory environment 6 Examination of plant and animal cells Preparation of materials such as Lame-Lamellae for the microscope Visual and practical education in laboratory environment 7 Examination of animal tissues Preparation of materials such as Lame-Lamellae for the microscope Visual and practical education in laboratory environment 8 Examination of animal limbs Supplying animal limbs (sheep heart, lung, brain and kidney, etc) Visual and practical education in laboratory environment 9 The animal dissection (mouse and frog) Supplying a frog and mouse Visual and practical education in laboratory environment 10 Examination of a living sperm and ovum cell Supplying frog ovum and testicle Visual and practical education in laboratory environment 11 Examination of giant chromosomes Supplying a giant chromosome from mosquitos´ salivary glands and malpighia tubules and painting them Visual and practical education in laboratory environment 12 Examination of human chromosomes Preparation of metaphasis plate obtained from human blood Visual and practical education in laboratory environment 13 DNA isolation from blood Supplying the required chemicals and solutions for human blood and DNA isolation Visual and practical education in laboratory environment 14 DNA isolation from tissue Supplying the required chemicals and solutions for human tissue and DNA isolation Visual and practical education in laboratory environment 15 The electrophoresis methods and agarose gel electrophoresis Supplying the required materials for electrophoresis Visual and practical education in laboratory environment 16/17 Final exam Exam preparations in the form of questions-answers Visual and practical education in laboratory environment   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Kasap H, Kasap M. Medical Biology Laboratory Guide. Kemal Matbaası, 128 p., Adana, 1988. Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 0 0     Homeworks/Projects/Others 6 100 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Explains theorical debates of three main areas (philosophy, sociology, psychology) in relation to today´s problems 5 2 Knows the functions of tissues and organ systems. 5 3 Knows the structure of the genetic material. 4 4 Knows the genetic diseases and the genes that cause genetic diseases and disorders. 4 5 Is able to conduct molecular and cytogenetic diagnosis analysis. 5 6 Knows what numerical and structural abnormalities of the autosomes and the sex chromosomes mean and explain them. 5 7 Is able to do DNA isolation from all types of tissue. 5 8 Is able to perform PCR. 1 9 Is able to perform and interpret agarose and polyacrylamide gel electrophoresis. 5 10 Is able to measure the DNA obtained from tissues using a spectrophotometer. 2 11 Is capable of interpreting and implementing QF-PCR technique. 2 12 Is able to cell culture from blood, amniotic, chorionic villus biopsy specimens, and obtain chromosome preparation. 2 13 Is able to recognize and interpret all human chromosomes from the preparations derived from chromosome karyotype analysis. 3 14 Learns how to write and comment on a scientific article. 2 15 Knows the structure of genes. 2 16 Knows the chromosome and gene mutations. 5 17 Knows patterns of inheritance (autosomal dominant, autosomal recessive, sex-linked dominant and recessive inheritance models). 1 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 10 140     Out of Class Study (Preliminary Work, Practice) 14 0 0 Assesment Related Works     Homeworks, Projects, Others 6 2 12     Mid-term Exams (Written, Oral, etc.) 0 0 0     Final Exam 0 0 0 Total Workload: 152 Total Workload / 25 (h): 6.08 ECTS Credit: 6