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Course Description Course Name : Recombinant DNA Technology Course Code : BT-519 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Assoc.Prof.Dr. NESLİHAN YEŞİM YALÇIN MENDİ Learning Outcomes of the Course : Knows the development of genetics, the basic structures of DNA and RNA. Knows gene isolation, gene cloning and the basic principles of DNA analysis. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : BT-519 Recombinant DNA Technology Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is to discuss the basic structures of DNA and RNA molecules forming the basis of molecular biology studies and which techniques are used to change these structures and their application areas. Course Contents : This lesson contains introduction to Landmarks in modern genetics, making recombinant DNA, DNA isolation, cutting and joining DNA, amplifying the recombinant DNA, cloning vectors, making a library, the analysis of cloned genes. Language of Instruction : Turkish Work Place : Classrooms and Biotechnology labs of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 DNA and RNA structure and function Literature review of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 2 Genetic code and protein synthesis Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 3 Analysis of gene expression Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 4 Regulation of gene expression Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 5 Isolation of nucleic acids Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 6 The basic concepts in recombinant DNA technology Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 7 Restriction enzymes and their uses Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 8 The basic principles of gene isolation Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 9 Gene cloning and PCR Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 10 Gene cloning vectors Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 11 Making genomic DNA library Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 12 Making cDNA library Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 13 RNA interference and uses Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 14 Nucleic acid hybridization Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 15 Gene cloning and DNA analysis in agriculture Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet 16/17 Assessment and discussion Examination of scientific papers and distribution of issues Powerpoint presentation and visual animations from internet   Required Course Resources Resource Type Resource Name Recommended Course Material(s)   Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 3 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Evaluates and directs his level of learning in the field of knowledge and skills with his expert level critically. 5 2 Transfers current developments in the field of his work, supporting them with quantitative and qualitative data, systematically to the area outside of the field, written, orally and visually. 5 3 Follows national and international publications and attends social interactions and scientific studies in international level, communicates in at least in one foreign language in order to share studies on international base. 4 4 Uses advanced information and communication technologies along with the required level of their computer software. 3 5 Uses the knowledge in his field for problem solving and / or practical skills in interdisciplinary studies. 4 6 Improves common knowledge accumulation concerning the Biotechnology in the frame of basic theory and practices. 4 7 Is aware of scientific, ethical and social values and handles research process with this frame. 5 8 Handles theories, hypothesis, opinions in the field of Biotechnology with an objective sceptic, logical, analytical manner and evaluates them in critical point of view. 4 9 Gains comprehensive information about natural and applied sciences and its limits with modern techniques and methods applied. 3 10 Improves and increases the knowledge to an expert level in the field of biotechnology 5 11 Understands the interdisciplinary interaction associated with biotechnology. 5 12 Integrates and interprets the knowledge from different disciplines by his expertrise in biology and generate new information 4 13 Analizes the problems encountered in the field of research methods. 3 14 Carries out a study requiring expertise in the field independently. 5 15 Developes new strategic approaches and takes resposibility for analitical solutions for unpredictable complicated problems encountered in applications related to biotechnology. 4 16 Demonstrates leadership in the required environment to solve problems associated with biotechnology. 4 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 3 15 45     Mid-term Exams (Written, Oral, etc.) 1 14 14     Final Exam 1 14 14 Total Workload: 157 Total Workload / 25 (h): 6.28 ECTS Credit: 6