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Course Description Course Name : Biotechnological Applications in Plant Breeding and Seed Production Course Code : BT-506 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. SAADET BÜYÜKALACA Learning Outcomes of the Course : Obtains theoretical and conceptual information about plant breeding, seed production and biotechnology. Knows biotechnological methods used in plant breeding and seed production and implements these technical and cultural methods in different studies. Knows theoretical and practical methods to use in researches. Knows how to put research results and theoretical information into practical studies. Improves methods using biotechnological techniques to solve present problems. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : To give information related to the use biotechnological methods in horticultural crops breeding and seed production Course Contents : Description of biotechnology, introduction to plant tissue culture ( plant tissue culture labs, environments, aseptic techniques), organogenesis, somatic embriyogenesis, somaclonal variation, haploidy, protoplast fusion and somatic hybridization, seconder metabolites, germplasm conservation, molecular markers, gene transfer, the importance of the quality of plant-seed, plant -seed production system in Turkey, plant-seed production regions, ecologic factors in plant-seed production, the principles of plant-seed production and biotechnological solutions to problems on plant-seed production and breeding of horticultural crops, the use of in vitro and molecular techniques in horticulture, genetic engineering applications in horticulture . Language of Instruction : Turkish Work Place : Post-graduate lecture room of Department   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Definition of plant biotechnology, history and general definition of the application areas Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 2 The definition of plant tissue cultures, objectives, steps and overall presentation of the usage areas in horticulture. Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 3 Features and configuration of plant tissue culture laboratory, presentation of basic tools, equipment and materials used in vitro studies. Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 4 Shoot tip, Meristem and micro-grafting techniques Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 5 Somatic embryogenesis Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 6 İn Vitro Haploid plant production techniques. Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 7 Mid-term exam Preparation to mid-term exam Written examination 8 Embryo and protoplast cultures and protoplast fusion. Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 9 Callus and cell cultures Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 10 Seconder metabolite production in vitro Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 11 Somaclonal variation, in vitro preservation of genetic materials. Reading recommended books and given lecture notes Lecturing, powerpoint presentation, discussion and lab application 12 The structure of DNA Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 13 Description and methods of gene transfer. Gene transfer in horticultural crops and obtention of tarnsgenic plants. Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 14 DNA markers. Hybridization and PCR-based markers. Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 15 Use of molecular markers in horticulture Reading recommended books and given lecture notes Lecturing, powerpoint presentation and discussion 16/17 Final examination Preparation to final exam Written examination   Required Course Resources Resource Type Resource Name Recommended Course Material(s)       Required Course Material(s)     Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 3 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Evaluates and directs his level of learning in the field of knowledge and skills with his expert level critically. 3 2 Transfers current developments in the field of his work, supporting them with quantitative and qualitative data, systematically to the area outside of the field, written, orally and visually. 3 3 Follows national and international publications and attends social interactions and scientific studies in international level, communicates in at least in one foreign language in order to share studies on international base. 3 4 Uses advanced information and communication technologies along with the required level of their computer software. 2 5 Uses the knowledge in his field for problem solving and / or practical skills in interdisciplinary studies. 4 6 Improves common knowledge accumulation concerning the Biotechnology in the frame of basic theory and practices. 4 7 Is aware of scientific, ethical and social values and handles research process with this frame. 5 8 Handles theories, hypothesis, opinions in the field of Biotechnology with an objective sceptic, logical, analytical manner and evaluates them in critical point of view. 3 9 Gains comprehensive information about natural and applied sciences and its limits with modern techniques and methods applied. 5 10 Improves and increases the knowledge to an expert level in the field of biotechnology 5 11 Understands the interdisciplinary interaction associated with biotechnology. 4 12 Integrates and interprets the knowledge from different disciplines by his expertrise in biology and generate new information 3 13 Analizes the problems encountered in the field of research methods. 3 14 Carries out a study requiring expertise in the field independently. 3 15 Developes new strategic approaches and takes resposibility for analitical solutions for unpredictable complicated problems encountered in applications related to biotechnology. 3 16 Demonstrates leadership in the required environment to solve problems associated with biotechnology. 2 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 3 10 30     Mid-term Exams (Written, Oral, etc.) 1 14 14     Final Exam 1 14 14 Total Workload: 142 Total Workload / 25 (h): 5.68 ECTS Credit: 6