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Course Description Course Name : Otolog bone marrow purging and freezing processes I Course Code : PH-550 Course Type : Optional Level of Course : First Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 5 Name of Lecturer(s) : Prof.Dr. YURDANUR KILINÇ Learning Outcomes of the Course : A student can perform aspiration of bone marrow of the patients. A student learns the techniques of selection of approppriate cells, freezing and storage-keeping them. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : When students complete "the purging and freezing processes of bone marrow cells" course, they will be able to collect, measure the viability of cells, monoclonal antibodies and complement syst and can do manipulations for processing. Course Contents : The usage of 4-HC in otologous bone marrow cells in purging, extracorporeal bone marrow cells, purging processes by photoirradiasyon, monoclonal antibodies and complement system, immunomagnetic manupulation of bone marrow cells, freezing processes of bone marrow, bone marrow preservation with Dimethyl sulphoxide and Hydroxyethyl starch, the selection of hematopoietic stem cells, genetic manipulations in bone marrow, gene transfer with retroviruses.. Language of Instruction : Turkish Work Place : Seminar Halls and laboratories of Pediaric Hematology, Blood Bank Apheresis Unit, Pediatric BMT Laboratories.   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Bone Marrow:anatomu, stromal and medulalary component Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 2 The origin and differentiation of the cells. Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 3 Granulocytes, monocytes and lymphocytes:specialties of the cells Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 4 The preparation of donor Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 5 The preparation of donor Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 6 Harvesting Process Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 7 Harvesting Process Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 8 Purging Processses:Selection and definition. Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 9 Purging Processses:Physical techniques. Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 10 Purging Processes:Chemical Techniques. Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 11 Purging Processes:Immunological Techniques. Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 12 Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 13 Cryopreservation Practice and Laboratory Work Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 14 Make-up Lesson Uygulama ve Laboratuvar Çalışması Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 15 Seminar Preparation from the sources given before, literature search Instruction, seminar, group discussion, brain storming. 16/17 Final Exam Revision of the sources Written/oral or applied exams   Required Course Resources Resource Type Resource Name Recommended Course Material(s)   Molecular Hematology: Provan D,and Gribben J(eds), Blackwell Scientific Co, Tokyo, 2000  The EBMT Handbook :Apperley J, Carreras E, Gluckman E, Gratwohl A, Masszi T(eds), The European School of Hematology EBMT Handbook, 5t ed, Saint Louis, 2008. Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 90     Homeworks/Projects/Others 2 10 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 A student can perform laboratory techniques. 4 2 A student can communicate and create bonds with the patients. 1 3 A student can teach the bone marrow stromal and medullary componants which are basic components of hematology. 4 4 A student can describe the rules of basic hematology. 3 5 A student can become trained in the area of molecular changes in benign and malign hematological disorders. 4 6 A students acknowledges blood cells biologies, blood and blood components transfusion and stem cell transplantation procedures. 4 7 A student gets prepared for stem cell transplant, collection, seperation, freezing topics. 4 8 A student can explain the structure and functions of erythrocytes, leucocytes and platelets. 4 9 A student can work in a pediatric hematology laboratory. 3 10 A student gets prepared for basic rules of gene therapy. 4 11 A student can investigate the molecular basis of benign and malign hematological disorders. 4 12 A student can explain the physiopathologies and results of hemostasis and thrombosis. 2 13 A student becomes competent in the application areas of blood cells and components and bone marrow transplantation 4 14 A student can perform application of the laboratory techniques necessary for pediatric hematology. 4 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 1 14 Assesment Related Works     Homeworks, Projects, Others 2 20 40     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 10 10 Total Workload: 130 Total Workload / 25 (h): 5.2 ECTS Credit: 5