DEGREE PROGRAMS  Associate's Degree (Short Cycle)  Bachelor’s Degree (First Cycle)  Master’s Degree (Second Cycle)

Course Description Course Name : Isolation Methods of Nucleic Acids and Storage of Them Products Course Code : BİK-551 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 5 Name of Lecturer(s) : Prof.Dr. ABDULLAH TULİ Prof.Dr. LEVENT KAYRIN Prof.Dr. KIYMET AKSOY Prof.Dr. NURTEN DİKMEN Prof.Dr. MEHMET AKİF ÇÜRÜK Assoc.Prof.Dr. TAMER C. İNAL Asst.Prof.Dr. ÖZLEM GÖRÜROĞLU ÖZTÜRK Learning Outcomes of the Course : describes the structural features of nucleic acids classifies nucleic acids discusses the effect of structural features of nucleic acids to obtaining them knows and applies the usage of nucleic acids in methods knows the solution that nucleic acids are solved prepares solutions calculates the product concentration discusses and applies product storage conditions Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : the aim of this course is to enable students learn different methods of obtaining nucleic acids and storing them in appropriate conditions Course Contents : structural features and classifications of nucleic acids. obtaining nucleic acids with different methods. the importance of structural features in obtaining nucleic acids. calculation of concentration. factors that effect product efficiency and quality. features and preparation of solutions, conditions for storage and application Language of Instruction : Turkish Work Place : classes and laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 introduction to nucleic acids; definition, classification and structural features reading related sources interactive and practice 2 introduction to nucleic acids; definition, classification and structural features reading related sources interactive and practice 3 methods for obtaining nucleic acids reading related sources interactive and practice 4 methods for obtaining nucleic acids reading related sources interactive and practice 5 methods for obtaining nucleic acids reading related sources interactive and practice 6 methods for obtaining nucleic acids reading related sources interactive and practice 7 effect of structural features of nucleic acids to obtaining nucleic acids reading related sources interactive and practice 8 mid-term examination revision of previous lessons mutual discussion 9 eficiency and specificity of nucleic acid products reading related sources interactive and practice 10 molecular methods for obtaining products related to nucleic acid reading related sources interactive and practice 11 molecular methods for obtaining products related to nucleic acid reading related sources interactive and practice 12 solutions for solving nucleic acids and their characteristics reading related sources interactive and practice 13 calculating the product concentration reading related sources interactive and practice 14 product storage conditions reading related sources interactive and practice 15 discussion revision of previous lessons brain storming 16/17 Final examination revision of lessons mutual brain storming   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 3rd Ed., Cold Spring Harbor Laboratory Press, 2001.  Dieffenbach CW, Dveksler GS. PCR Primer: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, 2003  White BA. PCR Protocols: Current Methods and Applications, Humana Press, 1998. Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 50     Homeworks/Projects/Others 5 50 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Applies innovations in laboratory processes by doing research. 3 2 Comprehends the interaction of biochemistry with the other scientific fields. 1 3 Knows experimental methods in biochemistry. 4 4 Takes part in in-vitro/in-vivo animal research studies. 0 5 Carries out studies in the field of biochemistry as a member of a team. 1 6 Gains ongoing professional skills. 3 7 Comprehends basic information about the field of biochemistry. 2 8 Interprets the factors and phases that effect the laboratory results. 2 9 Uses laboratory tools. 4 10 Knows the ethical and legal characteristics of laboratory processes, and interpretes economical value of these processes for the country. 2 11 Supports a scientific research. 3 12 Interpretes scientific research results which are carried out. 3 13 Generates a report from the conclusion of the scientific research. 3 14 Knows biochemical sampling rules. 4 15 Studies and makes research in laboratory settings. 3 16 Corporates with the other members of the team in an efficient, sensitive and respectfull way. 2 17 Interpretes and corrolates biochemical data and theoretical concept. 1 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 2 28     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 5 5 25     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 10 10 Total Workload: 115 Total Workload / 25 (h): 4.6 ECTS Credit: 5