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Course Description Course Name : Laboratory Technics of Plant Biotechnology Course Code : BB-595 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. YILDIZAKA KAÇAR Learning Outcomes of the Course : Learns the preparation of solutions used in plant biotechnology laboratory. Learns the basic techniques of tissue culture. Understands PCR applications. Knows the techniques of gene transfer. Gains experience in laboratory safety. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : The aim of this course is to examine the main techniques applied in plant biotechnology laboratory. Course Contents : The contents of this course include the definition and application of plant biotechnology, solution preparation, microscope, centrifuge, use of PHmeter in biotechnology, plant tissue cultures, the preparation of liquid-solid LB medium, creation of a single colony of bacteria, Agrobacterium tumefaciens-mediated gene transfer, DNA isolation and quantification, PCR applications, agarose gel electrophoresis, polyacrylamide gel electrophoresis, DNA sequence analysis. Language of Instruction : Turkish Work Place : Classrooms and Plant biotechnology Lab. of Department of Horticulture, Faculty of Agriculture, University of Cukurova   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Definition and application of biotechnology of plant biotechnology Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 2 Preparation of the solutions Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 3 Use of microscope, centrifuge, PH meter in biotechnology Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 4 Conditions laboratory of plant tissue cultures and sterilization Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 5 Plant tissue cultures Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 6 Preparation of liquid-solid LB medium, creation of a single colony of bacteria Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 7 Agrobacterium tumefaciens mediated gene transfer Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 8 DNA isolation and determination of quality and quantity Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 9 RNA and mRNA isolation, cDNA synthesis and determination of the quality-quantity Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 10 Mid-Term Exam Preparation of exam Written exam 11 PCR Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 12 Agarose gel electrophoresis Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 13 Polyacyrilamide gelelectrophoresis Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 14 DNA bands imaging methods in polyacrylamide gel Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 15 DNA sequencing Reading the related lecture notes Straight narrative technique, power point presentation, discussion and lab activities 16/17 Final Exam Preparation of the exam Written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Handbook of Plant Biotechnology John Wiley and Sons Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 70     Homeworks/Projects/Others 4 30 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications 2 2 Understanding the horticulture and the interaction between the related disciplines 1 3 Solving problems by research methods and establishing cause-effect relationship 3 4 Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. 2 5 Carrying out a study individually that requires professional knowledge in Horticulture 2 6 Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results 2 7 Leading in the situations when solving problems related to horticulture is necessary 1 8 Entering database related to horticulture, utilizing those sources and self-updating. 2 9 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process 1 10 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments 5 11 Using software related to horticulture sufficiently and using information and communication technologies in advanced level 4 12 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values 3 13 Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating 2 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 2 28 Assesment Related Works     Homeworks, Projects, Others 4 6 24     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 20 20 Total Workload: 138 Total Workload / 25 (h): 5.52 ECTS Credit: 6