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| Course Description |
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| Course Name |
: |
Applied Biotechnology In Fruit Trees |
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| Course Code |
: |
BK-590 |
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| Course Type |
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Optional |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
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| Course Semester |
: |
Spring (16 Weeks) |
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| ECTS |
: |
6 |
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| Name of Lecturer(s) |
: |
Prof.Dr. MUKADDES KAYIM
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| Learning Outcomes of the Course |
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Learns principles of plant biotechnology
Learns vitro clonal production of fruit trees by tissue culture methods
Learns shoot-tip grafting in fruit trees
Learns genetic transformation of woody plants and transgenic plant analysis
Learns application of Protoplast culture and fusion techniques in trees
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| Mode of Delivery |
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Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
BK-501 Bioenergetics
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| Recommended Optional Programme Components |
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None |
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| Aim(s) of Course |
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Teaching tissue culture methods in fruit trees such as cells, meristem and tissue culture, somatic hybridization, gene transfer and analysis of GMO by theoretical and practical application. |
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| Course Contents |
: |
This course covers all plant biotechnological methods such as tissue and meristem culture, vitro clonal production, somaclonal variation, shoot-tip grafting, protoplast culture, somatic hybridization, gene transfer methods in citrus, apple, nectarin, apricot, cherry, pomegranate, olive tees. Besides, biotechnologically produced plant materials will be analysed by application. |
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| Language of Instruction |
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Turkish |
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| Work Place |
: |
Classroom,Laboratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
Introduction to chronological progress in plant biotechnology and charging in graduate students with a specific subject |
Literature revew |
lecture |
|
2 |
Tissue and meristem culture, and shoot-tip grafting in citrus trees and application. |
Literature revew |
lecture |
|
3 |
In vitro clonal production in rootstocks of stone fruit trees (nectarines, apricot, cherry) and application fields |
Literature revew |
lecture |
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4 |
In vitro clonal production and application in olive trees |
Literature revew |
lecture |
|
5 |
Protoplast culture in citrus and application fileds. |
Literature revew |
lecture and laboratory study |
|
6 |
Protoplast fusion in citrus and application |
Literature revew |
lecture and laboratory study |
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7 |
Screening of somatic hybrids |
Literature revew |
lecture and laboratory study |
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8 |
Midterm exam |
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Exam |
|
9 |
Gene transfer into citrus rootstocks and application |
Literature revew |
lecture and laboratory study |
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10 |
Gene transfer into citrus protoplast and application fileds |
Literature revew |
lecture and laboratory study |
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11 |
In planta transformation |
Literature revew |
lecture and laboratory study |
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12 |
Genetic transformation of some trees and application |
Literature revew |
lecture and laboratory study |
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13 |
GMO analysis |
Literature revew |
lecture and laboratory study |
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14 |
Analysis of somatic hybrids |
Literature revew |
lecture and laboratory study |
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15 |
Presentation of homework and discussion |
Literature revew |
lecture and laboratory study |
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16/17 |
Final exam |
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Exam |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Plant tissue culture: Basic and applied. 2005. Timir Baran Jha, Bisvajit Ghosha 57p.
Introduction to plant biotechnology Second edition. 2002. H.S. Chawla (Ed.)
Plant Biotechnology: Genetic manipulation of plants. 2003. Adrian Slater, Nigel W. Scott, Mark R. Fowler (Eds.). 346p.
Introduction to plant tissue culture. 2003. M.K. Razdan, 154 p.
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| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
80 |
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Homeworks/Projects/Others |
5 |
20 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
| |
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Final Assessments
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100 |
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Rate of Final Assessments to Success
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60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
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1 |
Has knowledge on basic topics about general science, general agricultural engineering and plant production |
5 |
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2 |
Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants |
4 |
|
3 |
Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice |
4 |
|
4 |
Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods |
5 |
|
5 |
Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly |
4 |
|
6 |
Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports |
5 |
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7 |
Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations |
5 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
|
Class Time (Exam weeks are excluded) |
14 |
4 |
56 |
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Out of Class Study (Preliminary Work, Practice) |
14 |
2 |
28 |
| Assesment Related Works |
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Homeworks, Projects, Others |
5 |
10 |
50 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
8 |
8 |
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Final Exam |
1 |
16 |
16 |
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Total Workload: | 158 |
| Total Workload / 25 (h): | 6.32 |
| ECTS Credit: | 6 |
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