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Course Description Course Name : Methods for the Diagnosis of Plant Pathogen Bacteria II Course Code : BK-570 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. YEŞİM AYSAN Learning Outcomes of the Course : Accesses practical information about the pathogenicity tests Learns serology and accesses practical information about the use of the diagnosis of pathogenic bacteria Learns molecular tests and has practical knowledge of how to make these tests Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : Aim of the lecture is learning bacterial identificaiton by serological and molecular methods. These methods are intended to learn in practice. Course Contents : In the identificaiton of bacterial strains ELISA, IF, BIOLOG, MIS, API and PCR methods Language of Instruction : Turkish Work Place : Clasroom and laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 General information about the process of identification of plant pathogenic bacteria Textbooks, supplementary books and presentations preparation lecture 2 The role of traditional tests in the identification of plant pathogenic bacteria (morphological, physiological and biochemical) Textbooks, supplementary books and presentations preparation lecture 3 Confirmation tests used in the identification of plant pathogenic bacteria Textbooks, supplementary books and presentations preparation lecture 4 Pathogenicity test applications and the importance of koch postulate Textbooks, supplementary books and presentations preparation lecture and laboratory study 5 Bacterial identification by serological methods Textbooks, supplementary books and presentations preparation lecture and laboratory study 6 ELISA test and uses Textbooks, supplementary books and presentations preparation lecture and laboratory study 7 Bacterial identification by protein and lipoloysaccaride analysis Textbooks, supplementary books and presentations preparation lecture and laboratory study 8 Exam Textbooks, supplementary books and presentations preparation exam 9 Detection of the bacteria from seed material by immunoflorescens mikroskopy methods Textbooks, supplementary books and presentations preparation lecture 10 Bacterial identification by whole cell fatty acid analysis (MIDI) Textbooks, supplementary books and presentations preparation lecture 11 Bacterial identification by API Textbooks, supplementary books and presentations preparation lecture 12 Microbial identificatiom methods based on nucleic acids Textbooks, supplementary books and presentations preparation lecture and laboratory study 13 PCR methods and usage Textbooks, supplementary books and presentations preparation lecture and laboratory study 14 Bacterial identification by phage, biolüminescent, flow cytometri applications Textbooks, supplementary books and presentations preparation lecture 15 Integrated bacterial identificaiton Textbooks, supplementary books and presentations preparation lecture 16/17 Final exam Textbooks, supplementary books and presentations preparation exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Laboratory Guide for Identification of Plant Pathogenic Bacteria (Third Edition) Edts:Schaad, N. W., Jones, J.B., and Chun, W.. APS Press. St. Paul, Minnesota  Methods for the diagnosis of bacterial diseases of plants. Lelliott and Stead, 1987.  Phytobacteriology: principles and practice. Janse, 2005.  Plant Bacteriology. Kado, 2010  Methods in Phytobacteriology. Edts: Klement, Rudolpf and Sands, 1990 Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 50     Homeworks/Projects/Others 5 50 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has knowledge on basic topics about general science, general agricultural engineering and plant production 3 2 Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants 3 3 Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice 5 4 Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods 4 5 Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly 3 6 Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports 5 7 Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 5 10 50     Mid-term Exams (Written, Oral, etc.) 1 6 6     Final Exam 1 10 10 Total Workload: 150 Total Workload / 25 (h): 6 ECTS Credit: 6