| Course Description |
|
| Course Name |
: |
Application of Biotechnological Methods in Mycology |
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| Course Code |
: |
BK-567 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
Second Cycle |
|
| Year of Study |
: |
1 |
|
| Course Semester |
: |
Fall (16 Weeks) |
|
| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. MUKADDES KAYIM
|
|
| Learning Outcomes of the Course |
: |
Learns how to prepare basic and selective media for plant fungal pathogenes
Learns nucleic acid extraction from plant fungal genome
Learns applied molecular methods in classification of plant fungal pathogenes
Learns the genes in plant fungal classification
Knows application of phylogenetics of fungal pathogenes and combination of the classical methods for classification
Knows classification of plant fungal pathogenes based on traditional and molecular methods and comparision of these methods
|
|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
BK-501 Bioenergetics
|
|
| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
The aim of this course is to teach biotechnological and molecular methods in plant fungal pathogenes, genes used in molecular diagnosis, PCR based protocols, phylogenetic analysis and comparing the results based on molecular and traditional methods (based on morphological apperance and physiological request) |
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| Course Contents |
: |
Introducing of fungi kingdom, classification, molecular characterization, phylogenetic analysis, molecular characterization of purified fungal pathogenes from plant, phylogenetic analysis, classification of fungal pathogenes based on gene groups. Application of biotechnology in fungal field. |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Classroom,Laboratory |
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|
Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
General characterization of plant fungal pathogenes |
Lecture notes and related books |
Visual |
|
2 |
Classification of plant pathogenes |
Lecture notes and related books |
Visual |
|
3 |
Overview of molecular methods in plant fungal pathogen diagnosis. Graduate students charge in homework for their interest. |
Lecture notes and related books,laboratory study results |
Laboratory practice and visual |
|
4 |
Application of Internal transcribed spacer gene (ITS) for plant fungal pathogen classification |
Lecture notes and related books,laboratory study results |
Laboratory practice |
|
5 |
Application Beta tubulin gene (TUB2) for plant fungal pathogen classification |
Lecture notes and related books |
Laboratory practice and visual |
|
6 |
Application of elongation factor 1 gene (EF1) for plant fungal pathogen classification |
Lecture notes and related books |
Laboratory practice and visual |
|
7 |
Application of small and large subunite genes (SSU and LSU) for plant fungal pathogen classification |
Lecture notes and related books |
Laboratory practice |
|
8 |
Internal exam |
Lecture notes and related books |
Wrıtting and oral exam |
|
9 |
Application of Actin (ACT)’ Calmodulin (CAL), Chitin syntase (CHS-1) genes for plant fungal pathogen classification, |
Lecture notes and related books |
Laboratory practice and visual |
|
10 |
Application of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glutamine synthase (GS) genes for plant fungal pathogen classification |
Lecture notes and related books |
Laboratory practice and visual |
|
11 |
Phylogenetic analysis of genes used in fungal classification -1 |
Lecture notes and related books |
Laboratory practice and visual |
|
12 |
Phylogenetic analysis of genes used in fungal classification-2 |
Lecture notes and related books |
Laboratory practice and visual |
|
13 |
Application of Phylogenetic analysis in fungal classification |
Lecture notes and related books |
Laboratory practice |
|
14 |
Application of Gen transfer for plant fungal pathogen |
Lecture notes and related books |
Laboratory practice |
|
15 |
Presentation of homework by graduate students and discussion |
Lecture notes and related books |
Laboratory practice and visual |
|
16/17 |
Final exam |
Lecture notes and related books |
Wrıtting and oral exam |
|
|
|
Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Phylogenetic trees made easy 4th edit:on Barry G. ll 2011
A phylogenetic re-evaluation of Dothideomycetes: Conrad L. Schoch, Joseph W. Spatafora, H. Thorsten Lumbsch, Sabine M. Huhndorf, Kevin D. Hyde, Johannes Z. Groenewald and Pedro W. Crous (Eds). Studies in Mycology 64, 2009.
Colletotrichum: complex species or species complexes? Ulrike Damm, Paul F. Cannon and Pedro W. Crous (Eds.) Studies in Mycology 73, 2012.
|
| |
| Required Course Material(s) |
Introductory Mycology1996. C.J. Alexopoulos, C.W. Mims, M. Blackwell (Eds.)
Plant Pathology George N. Agrios 4th Edition 2004
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|
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Has knowledge on basic topics about general science, general agricultural engineering and plant production |
5 |
|
2 |
Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants |
5 |
|
3 |
Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice |
4 |
|
4 |
Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods |
5 |
|
5 |
Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly |
3 |
|
6 |
Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports |
5 |
|
7 |
Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations |
5 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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