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Course Description Course Name : Serology in Phytopathology Course Code : BK-526 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Spring (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. SAADETTİN BALOĞLU Learning Outcomes of the Course : Learns how to use and investigate serological tests used commonly especially on the identification of virus diseases on phytopathoogy Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : Serology which is one of the important methods used for identification of especially viruses on phytopathology is also used in the identification of bacterial and fungal disease. As a reliable technique, serology is taught with the preparing compounds, applicating and investigating results.The uses of ELISA test and evaluation of the test results is also taught. Course Contents : Serology on phytopathology, determinant, serologic test, application of serologic tests, investigating of results. Language of Instruction : Turkish Work Place : Classroom and loboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 Introduction, Immunology and Serology Power point presentations, laboratory practice 2 Antigen and Antibodies Lecture documents, companion volume Power point presentations, laboratory practice 3 Viral and bacterial antigens Lecture documents, companion volume Power point presentations, laboratory practice 4 Polyclonal and monoclonal antibodies Lecture documents, companion volume Power point presentations, laboratory practice 5 Preparing of antigen, virus purification, preparing antigen from bacteria Lecture documents, companion volume Power point presentations, laboratory practice 6 Preparing of polyclonal antibody Lecture documents, companion volume Power point presentations, laboratory practice 7 Preparing of monoclonal antibody Lecture documents, companion volume Power point presentations, laboratory practice 8 Midterm exam Lecture documents, companion volume Written exam 9 Conjugating of antibodies Lecture documents, companion volume Power point presentations, laboratory practice 10 Test with Liquid phase, micro precipitating, and ring interphase Lecture documents, companion volume Power point presentations, laboratorypractice 11 Test with Liquid phase, agar diffusion tests, single double Lecture documents, companion volume Power point presentations, laboratory practice 12 Density gradient tests, immune electrophoresis tests Lecture documents, companion volume Power point presentations, laboratory practice 13 Tests with solid phase, preparing of ELISA kits Lecture documents, companion volume Power point presentations, laboratory practice 14 Application of ELISA tests, using and investigating, Lecture documents, companion volume Power point presentations, laboratory practice 15 other tests Lecture documents, companion volume Power point presentations, laboratory practice 16/17 Final exam All lecture documents Written examination   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Hampton,R., Ball, E., De Boer , S. 1990. Serological Methoda for Detection and ıdentification of Viral and Bacterial Plant Pathogens. A Laboratory Manual. The American Phthopathological Sociewty 3340 Pilot Knob Road, St Paul, Minnesota 55121-USA Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 50     Homeworks/Projects/Others 3 50 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has knowledge on basic topics about general science, general agricultural engineering and plant production 4 2 Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants 0 3 Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice 5 4 Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods 0 5 Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly 0 6 Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports 5 7 Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 3 12 36     Mid-term Exams (Written, Oral, etc.) 1 8 8     Final Exam 1 10 10 Total Workload: 138 Total Workload / 25 (h): 5.52 ECTS Credit: 6