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Course Description Course Name : DNA Cloning and Preparation of Gene Libraries Course Code : BY-611 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Prof.Dr. HATİCE KORKMAZ GÜVENMEZ Learning Outcomes of the Course : Understands gene cloning and the importance of DNA manipulations Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : To make an inquiry principles of DNA cloning and constructions of gene libraries Course Contents : Gene cloning and basic principle of DNA analysıs; Why important gene cloning and DNA analysıs, Discovery of gene cloning and PCR, What is the gene cloning What is the PCR? And importance Gene isolation with PCR Vectors for gene cloning (plasmids and bacteriophages). Purification of DNA from live cells. Manipulations of pured DNA. Intake the DNA to live cells. Cloning vectors for eukaryotes To determine one clone from gene library. The methods for determining clones. Gene libraries. Language of Instruction : Turkish Work Place : office or classroom   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 The basic principles of gene cloning and DNA analysis. scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 2 Why important gene cloning and DNA analyses scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 3 Discovery of gene cloning and PCR, scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 4 .What is the gene cloning? scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 5 What is the PCR and importance scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 6 Gene isolation with PCR scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 7 Vectors for gene cloning (plasmids and bacteriophages) scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 8 mid-term exam 9 Purification of DNA from live cells scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 10 Manipulations of pured DNA scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 11 Intake the DNA to live cells scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 12 Cloning vectors for eukaryotes scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 13 To determine one clon from gene library scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 14 The methods for determining clones scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 15 Gene libraries scan-to-date course notes and articles telling with lecture notes, presentation with projection, and interactive discussion 16/17 final exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  1-Gene cloning and DNA analyses, Brown, T. 2006. 2-DNA Science,Micklos, D.A. and et al.,2009. Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 80     Homeworks/Projects/Others 1 20 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Has the advanced hypothetical and applied knowledges in biology field 3 2 Based on the bachelor´s level of qualification, develops and deepens the knowledge as a specialist in the biology 4 3 Uses the theoretical and practical knowledge he expertises in biology 4 4 Integrates and interprets the knowledge from different disciplines by his expertise in biology and generates new information. 3 5 Solves the problems of biology by using research methods. 3 6 Carries out an independent study requires expertise on biology 4 7 Develops and produces new strategic approaches and solves the unexpected problems in biology area by taking responsibilty 5 8 Assesses the knowledge he expertises through critical approach and makes leadership solving problems related to biological environments. 4 9 Systematically transfers the current developments in the field of biology and his/her studies to the person in biology field or other related fields by supporting qualitative and quantitative data 3 10 Uses at least one foreign language to keep up to date with the field to take part in scientific and social interaction groups to share information at international levels 4 11 Uses information and communication technologies as required in the biology field 1 12 In biology, uses statistical software programme to carry out scientific research and also uses information and communication technology in proficiency level of knowledge and skills to critically evaluate 1 13 Develops the strategy, policy and implementation plans in the issues related to the field of biology and treats the results obtained within standart of quality 4 14 Uses the knowledge, problem solving, and / or practical skills obtained in the Biology Field in interdisciplinary studies. 3 15 Oversees social, scientific, cultural and ethical values in order to collect, implement, interpret data in the Biology area 5 16 Has the ability to formulate and solve the problems of biology in advanced level 5 17 Has ability to use his advanced knowledge in biology field for thinking systematically and solve problems in multidisciplinary approaches 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 3 42     Out of Class Study (Preliminary Work, Practice) 14 3 42 Assesment Related Works     Homeworks, Projects, Others 1 20 20     Mid-term Exams (Written, Oral, etc.) 1 20 20     Final Exam 1 30 30 Total Workload: 154 Total Workload / 25 (h): 6.16 ECTS Credit: 6