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  Course Description
Course Name : ELECTROPHORETIC DETERMINATION OF ISOENZYMES

Course Code : BİK-510

Course Type : Optional

Level of Course : Second Cycle

Year of Study : 1

Course Semester : Fall and Spring (16 Weeks)

ECTS : 4

Name of Lecturer(s) : Prof.Dr. ABDULLAH TULİ
Prof.Dr. LEVENT KAYRIN

Learning Outcomes of the Course : discribes enzyme and isoenzyme
owns knowledge about enzyme kinetics
knows the discribtion and the theory of electrophoresis
knows the usage and application field of enzyme and isoenzymes by electrophoesis and interprets specific samples of the issue
owns knowledge about the other application fields of electrophoresis and gel scanning

Mode of Delivery : Face-to-Face

Prerequisites and Co-Prerequisites : BİK-501 BASIC BIOCHEMISTRY I

Recommended Optional Programme Components : None

Aim(s) of Course : the aim of the course is to determine enzymes and isoenzymes by electrophoretic methods and their application fields

Course Contents : Enzyme and isoenzyme, enzyme kinetics, main definition and theory of electrophoresis, application fields of enzyme and isoenzymes by using electrophoresis and specific samples of this issue

Language of Instruction : Turkish

Work Place : Classroom


  Course Outline /Schedule (Weekly) Planned Learning Activities
Week Subject Student's Preliminary Work Learning Activities and Teaching Methods
1 Enzyme and isoenzyme listening and signification, preparing slides, interpreting the cases, critical thinking, improving questions lecturing
2 Enzyme and isoenzyme listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions lecturing
3 Enzyme and isoenzyme listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive, assignment, discussion
4 Enzyme kinetics listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive, laboratory, assignment
5 Enzyme kinetics listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive
6 Enzyme kinetics listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive, homework, discussion
7 General Definition of Electrophresis listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive
8 General Definition of Electrophresis listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive
9 Mid-term Examination study about the previous lessons classic
10 Sample Studies for Enzymes (Alkaline Phosphatase) listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive and application with case discussion
11 Sample Studies for Enzymes (Lactate Dehydrogenase) listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive and application with case discussion
12 Sample Studies for Enzymes (G6PD) listening and signification, preparing, slides, interpreting the cases, thinking judgemental, improving questions interactive and application with case discussion
13 Sample Studies for Enzymes (Pyruvate Kinase) listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive and application with case discussion
14 Sample Studies for Enzymes (Creatine Kinase) listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive and application with case discussion
15 Gel Scanning listening and signification, preparing slides, interpreting the cases, critical thinking , improving questions interactive and with laboratory application
16/17 Final Examination study about the previous lessons classic


  Required Course Resources
Resource Type Resource Name
Recommended Course Material(s)  Williams BL, Wilson Keith. Principles and Techniques of Practical Biochemistry, Edward Arnold Ltd., 1975.
 Segel IH. Enzymes, Chapter 4, in Biochemical Calculations, 2nd Ed., John Wiley & Sons, 1976.
 Burtis CA, Achwood ER. Tietz Textbook of Clinical Chemistry, 3rd Ed., Saunders, Philadelphia, 1996.
Required Course Material(s)


  Assessment Methods and Assessment Criteria
Semester/Year Assessments Number Contribution Percentage
    Mid-term Exams (Written, Oral, etc.) 1 50
    Homeworks/Projects/Others 4 50
Total 100
Rate of Semester/Year Assessments to Success 40
 
Final Assessments 100
Rate of Final Assessments to Success 60
Total 100

  Contribution of the Course to Key Learning Outcomes
# Key Learning Outcome Contribution*
1 Applies innovations in laboratory processes by doing research. 1
2 Comprehends the interaction of biochemistry with the other scientific fields. 4
3 Knows experimental methods in biochemistry. 2
4 Takes part in in-vitro/in-vivo animal research studies. 3
5 Carries out studies in the field of biochemistry as a member of a team. 0
6 Gains ongoing professional skills. 4
7 Comprehends basic information about the field of biochemistry. 4
8 Interprets the factors and phases that effect the laboratory results. 1
9 Uses laboratory tools. 2
10 Knows the ethical and legal characteristics of laboratory processes, and interpretes economical value of these processes for the country. 1
11 Supports a scientific research. 2
12 Interpretes scientific research results which are carried out. 2
13 Generates a report from the conclusion of the scientific research. 2
14 Knows biochemical sampling rules. 2
15 Studies and makes research in laboratory settings. 0
16 Corporates with the other members of the team in an efficient, sensitive and respectfull way. 0
17 Interpretes and corrolates biochemical data and theoretical concept. 2
* Contribution levels are between 0 (not) and 5 (maximum).

  Student Workload - ECTS
Works Number Time (Hour) Total Workload (Hour)
Course Related Works
    Class Time (Exam weeks are excluded) 14 1 14
    Out of Class Study (Preliminary Work, Practice) 14 1 14
Assesment Related Works
    Homeworks, Projects, Others 4 10 40
    Mid-term Exams (Written, Oral, etc.) 1 10 10
    Final Exam 1 20 20
Total Workload: 98
Total Workload / 25 (h): 3.92
ECTS Credit: 4