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Course Description Course Name : Techniques of Light Microscopy I Course Code : HİS512 Course Type : Compulsory Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Asst.Prof.Dr. HÜLYA ÖZGÜR Prof.Dr. ÖZGÜL TAP Learning Outcomes of the Course : Knows how to use a light microscobe. Prepares solutions for tissue fixation. Applies fixation techniques. Applies dehydration and clearing of a tissue section. Be able to embed the tissue sample appropriately. Applies sectioning from a microtome and places the tissue section on a slide. Applies staining methods. Takes photos of the specimen on light microscope. Offers solutions to problems at any technique step of the staining and preparation method. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : HİS502 General features of the tissues Recommended Optional Programme Components : None Aim(s) of Course : Aim of this course to learn and apply tissue preparation techniques to investigate organs and tissues under light microscope. Course Contents : Microscope types, Obtaining tissues, Fixation, Dehydration, Infiltration, Embedding, Sectioning, Staining, Decalsification Language of Instruction : Turkish Work Place : Lecture room, laboratory   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 General information about the methods of histological examination Self-study, reading recommended soruces Presentation 2 Microscope types and usage areas Self-study, reading recommended soruces Presentation and demonstration 3 Obtaining the tissues Self-study Presentation and demonstration 4 Fixation of the tissues Self-study Presentation and demonstration 5 Dehydration, clearing and embedding Self-study Presentation and demonstration 6 Cutting sections with microtome Self-study Presentation and demonstration 7 Basic principles in staining. General tissue staining methods. Hematoxylin-Eosin staining Self-study Presentation and demonstration 8 Midterm exam Self-study, reading recommended soruces Exam 9 Connective tissue dyes: Mallory Azan Self-study Presentation and application 10 Connective tissue dyes:Masson´s trichrome Self-study Presentation and application 11 Decalsification method in bone Self-study Presentation and demonstration 12 Fixation and staining of adipose tissue Self-study Presentation and demonstration 13 Smear preparation method Self-study Presentation and demonstration 14 Nerve tissue staining techniques Self-study Presentation and application 15 Abnormalities in light microscopic preparation technique Self-study Presentation and demonstration 16/17 Final exam Self-study, reading recommended soruces Exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Demir R.:Histolojik boyama teknikleri. Palme yayıncılık. Ankara, 2001.  Kaya,M. et al. Genel Histoloji Ders Notları. Ç.Ü. Tıp Fakültesi Yayınları. No:10, 2005.  Luna LG.: Manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. Mc Graw Hill book co. 1968. Required Course Material(s)  Lecture presentations   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 3 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Tell the main histological features of cells and tissues. 0 2 Corelate the structure and function of cells. 0 3 Have knowledge of the histologic features of organ systems. 0 4 Have knowledge of embryo development, developmental disorders and the mechanisms. 0 5 Define the methods of light and electron microscopic examination and tissue preparation. 5 6 Have knowledge of general principles about cell culture, histochemistry and immunohistochemistry. 0 7 Prepare solutions and tissues for light and electron microscopic studies . 5 8 Apply the basic methods of staining. 0 9 Distinguish tissue and organ systems in light microscopic level. 0 10 Perform studies as a team member or indıvidual. 4 11 Perform tasks which is given in research projects. 4 12 Develop positive attitudes and behaviors towards lifelong learning. 0 13 Determine and direct needs and resources of learnings by using resources effectively. 0 14 Evaluate knowledges and skills of the master degree critically. 0 15 Communicate effectively. 0 16 Relay ideas and solution offers about current developments in studies by supporting them with quantitative and qualitative data visually and verbally. 0 17 Know the importance of the ethical principles in research studies, evaluations and results. 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 5 70     Out of Class Study (Preliminary Work, Practice) 14 4 56 Assesment Related Works     Homeworks, Projects, Others 3 8 24     Mid-term Exams (Written, Oral, etc.) 1 2 2     Final Exam 1 2 2 Total Workload: 154 Total Workload / 25 (h): 6.16 ECTS Credit: 6