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Course Description Course Name : Bacterial Genetics Course Code : BİO511 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall and Spring (16 Weeks) ECTS : 5 Name of Lecturer(s) : Prof.Dr. DAVUT ALPTEKİN Learning Outcomes of the Course : Has knowledge about bacteria. Knows the structure of the bacterial cell. Knows the importance of bacteria in nature. Knows what transformation is. Knows what transduction is. Knows what conjugation is. Knows lytic and lysogenic life cycle of bacteria. Knows the structure and importance of viruses and bacteriophages. Knows transposons and the importance of transposons. Knows the recombinant DNA technology. Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : To teach students the structure of the bacteria and its importance in nature, the importance of bacterial genome, genetic modifications with transformation, transduction and conjugation, the importance of recombinant DNA technology, the importance of transposons and their effects on the human genome. Course Contents : General information about the bacteria. Bacterial cell structure, cell wall, cell membrane. Bacterial cytoplasm, intra cytoplasmic structures. Bacterial surface extensions, cillia, flagel, pilus. Bacterial media preparation. Bacterial genome: DNA, RNA. Regulation of gene functions in bacteria, operon theory. Mutations in bacteria. Bacteriophages, their morphology and taxonomy. Life cycles of phages. Transfer of genetic material between bacteria, Transformation. Transfer of genetic material between bacteria, Conjugation. Transfer of genetic material between bacteria, Transduction. Plasmids. Transposons. Language of Instruction : Turkish Work Place : Seminar room   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 General information about the bacteria. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 2 Bacterial cell structure, cell wall, cell membrane. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 3 Bacterial cytoplasm, intra cytoplasmic structures. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 4 Bacterial surface extensions, cillia, flagel, pilus. Bacterial media preparation. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 5 Bacterial genome: DNA, RNA. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 6 Regulation of gene functions in bacteria, operon theory. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 7 Mutations in bacteria. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 8 Bacteriophages, morphology, taxonomy. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 9 Llife cycles of phages. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 10 Midterm exam Preparation of multiple-choice questions. Multiple-choice test, oral exam. 11 Transfer of genetic material between bacteria, Transformation. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 12 Transfer of genetic material between bacteria, Conjugation. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 13 Transfer of genetic material between bacteria, Transduction. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 14 Plasmids. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 15 Transposons. Listening and signification, slide preparation, processing exceptions, critical thinking, question development. Standard classroom technologies, multimedia equipments, computers. 16/17 Final exam Preparation of multiple-choice questions. Multiple-choice test, oral exam.   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Akman M. Bacterial Genetics, Theoretical-Practical. Cumhuriyet University, Publication Number:1, Sivas, 1977.  Kasap H, Kasap M, Demirhan O, Alptekin D, Lüleyap Ü, Pazarbaşı A, Güzel Aİ. Medical Biology and Genetics. Ed. Halil Kasap, Nobel Tıp Kitabevi, Adana, 2010. Required Course Material(s)  Cooper GM, Hausman RE. The Cell, A Molecular Approach. mHücre Moleküler Yaklaşım. Third Edition, ASM Press, Washington,D.C. (USA), 1996.   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 60     Homeworks/Projects/Others 3 40 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Explains theorical debates of three main areas (philosophy, sociology, psychology) in relation to today´s problems 5 2 Knows the functions of tissues and organ systems. 0 3 Knows the structure of the genetic material. 5 4 Knows the genetic diseases and the genes that cause genetic diseases and disorders. 3 5 Is able to conduct molecular and cytogenetic diagnosis analysis. 2 6 Knows what numerical and structural abnormalities of the autosomes and the sex chromosomes mean and explain them. 0 7 Is able to do DNA isolation from all types of tissue. 2 8 Is able to perform PCR. 2 9 Is able to perform and interpret agarose and polyacrylamide gel electrophoresis. 0 10 Is able to measure the DNA obtained from tissues using a spectrophotometer. 0 11 Is capable of interpreting and implementing QF-PCR technique. 0 12 Is able to cell culture from blood, amniotic, chorionic villus biopsy specimens, and obtain chromosome preparation. 1 13 Is able to recognize and interpret all human chromosomes from the preparations derived from chromosome karyotype analysis. 0 14 Learns how to write and comment on a scientific article. 4 15 Knows the structure of genes. 2 16 Knows the chromosome and gene mutations. 2 17 Knows patterns of inheritance (autosomal dominant, autosomal recessive, sex-linked dominant and recessive inheritance models). 0 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 14 4 56     Out of Class Study (Preliminary Work, Practice) 14 4 56 Assesment Related Works     Homeworks, Projects, Others 3 4 12     Mid-term Exams (Written, Oral, etc.) 1 1 1     Final Exam 1 1 1 Total Workload: 126 Total Workload / 25 (h): 5.04 ECTS Credit: 5