DEGREE PROGRAMS  Associate's Degree (Short Cycle)  Bachelor’s Degree (First Cycle)  Master’s Degree (Second Cycle)

Course Description Course Name : Molecular Biological Methods in Aquaculture Course Code : ST-555 Course Type : Optional Level of Course : Second Cycle Year of Study : 1 Course Semester : Fall (16 Weeks) ECTS : 6 Name of Lecturer(s) : Asst.Prof.Dr. MAKBULE YANGÜN BAYLAN Learning Outcomes of the Course : Ensures being able to do studying entirely on one’s own for people wanting to join a study in genetics and biotechnology in aquaculture Mode of Delivery : Face-to-Face Prerequisites and Co-Prerequisites : None Recommended Optional Programme Components : None Aim(s) of Course : be teached and introduced of molecular biological methods used in aquaculture Course Contents : DNA isolation and analysis (DNA exraction of bacteria yeast and fish), Bacterial transformation (Chemical transformation, transformation by electroporation (electrotransformation)), DNA amplification by Polymerase Chain Reaction (PCR), isolation of proteins, purification and enzymatic analysis of proteins. Language of Instruction : Turkish Work Place : Classrooms of Ç.Ü.Faculty of Fisheries   Course Outline /Schedule (Weekly) Planned Learning Activities Week Subject Student's Preliminary Work Learning Activities and Teaching Methods 1 DNA isolation and analysis, Chromosome DNA isolation Reading at lecture note Oral presentation and powerpoint presentation 2 Chromosomal DNA isolation from bacteria Reading at lecture note Oral presentation and powerpoint presentation 3 Chromosomal DNA isolation from yeast Reading at lecture note Oral presentation and powerpoint presentation 4 Chromosomal DNA isolation from fish Reading at lecture note Oral presentation and powerpoint presentation 5 transformation by chemical aplication in bacteria Reading at lecture note and giving homeworks Oral presentation and powerpoint presentation 6 transformation by electroporation in bacteria Reading at lecture note Oral presentation and powerpoint presentation 7 DNA amplification by Polymerase Chain Reaction (PCR) Reading at lecture note Oral presentation and powerpoint presentation 8 midterm exam preparation for term exam written exam 9 formation mechanism of Polymerase Chain Reaction, basic components of PCR, functioning of PCR Reading at lecture note Oral presentation and powerpoint presentation 10 Amplification of correct pruduct with PCR, identification and analysis of PCR products Reading at lecture note Oral presentation and powerpoint presentation 11 prevention from contamination in PCR, types of PCR Reading at lecture note Oral presentation and powerpoint presentation 12 Protein isolation, purification and enzymatic analysis Reading at lecture note Oral presentation and powerpoint presentation 13 Tools, materials and chemicals Reading at lecture note and giving a presentation of homeworks Oral presentation and powerpoint presentation 14 Preparation of protein extract Reading at lecture note Oral presentation and powerpoint presentation 15 Protein extraction, storage conditions, extraction of fish tissue Reading at lecture note Oral presentation and powerpoint presentation 16/17 Final exam preparation for final exam written exam   Required Course Resources Resource Type Resource Name Recommended Course Material(s)  Temizkan G., Arda N., 2004. Using Methods in Molecular Biology , Nobel Kitapevi, 613p Required Course Material(s)   Assessment Methods and Assessment Criteria Semester/Year Assessments Number Contribution Percentage     Mid-term Exams (Written, Oral, etc.) 1 70     Homeworks/Projects/Others 1 30 Total 100 Rate of Semester/Year Assessments to Success 40   Final Assessments 100 Rate of Final Assessments to Success 60 Total 100   Contribution of the Course to Key Learning Outcomes # Key Learning Outcome Contribution* 1 Improves theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences. 3 2 Comprehends interactions between Fisheries Basic Sciences and other disciplines. 5 3 Determines strategies and investigates methods about their field of study in Fisheries Basic Science. 4 4 Produces new information and theories by interpreting and synthesising the information from other disciplines and uses the theoretical and practical information from their field of study in Fisheries Basic Science. 5 5 Collects data, interprets results and suggests solutions by using dialectic research methodology in the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. 5 6 Independently plans, designs and performs a certain project in the field of Fisheries Basic Sciences. 4 7 Produces solutions by improving new strategic approaches and taking responsibilities for the potential problems in the field of study as an individual or team member. 5 8 Determines the requirements for Fishery Basic Science education, reaches the resources, critically interpretes knowledge and skills and gains experience to direct the education. 5 9 Has positive stance on the lifelong education and uses it for the public benefit by using the gained theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences. 4 10 Follows the current topics and improvements in the field of Fisheries Basic Sciences, publishes and presents the research results, contributes to constitution of a public conscience in the field of interest. 5 11 Effectively communicates about the field of Marine and Inland Water Biology and Fisheries Basic Sciences by using written and oral presentation tools, follows up and criticizes the meetings and seminars. 5 12 Follows up international publications and communicates with international collaborators by using language skills. 4 13 Uses the communication and information technologies about the field of interest in an advanced level. 4 14 Conforms, controls and teaches social, cultural and scientific ethics in the investigation and publication process of the data related with the field of interest. 4 15 Improves strategies, politics and application codes by following scientific and technological developments on the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. Investigates and extends the results on behalf of public in frame of total quality management process. 3 16 Uses the abilities and experiences on applications and solving problems that gained during the MSc education for the interdisciplinary studies. 5 * Contribution levels are between 0 (not) and 5 (maximum).   Student Workload - ECTS Works Number Time (Hour) Total Workload (Hour) Course Related Works     Class Time (Exam weeks are excluded) 15 4 60     Out of Class Study (Preliminary Work, Practice) 15 3 45 Assesment Related Works     Homeworks, Projects, Others 1 8 8     Mid-term Exams (Written, Oral, etc.) 1 10 10     Final Exam 1 16 16 Total Workload: 139 Total Workload / 25 (h): 5.56 ECTS Credit: 6