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| Course Description |
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| Course Name |
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Basic Principles of Gene Cloning |
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| Course Code |
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BT-505 |
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| Course Type |
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Optional |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
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| Course Semester |
: |
Fall (16 Weeks) |
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| ECTS |
: |
6 |
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| Name of Lecturer(s) |
: |
Prof.Dr. BURHAN ARIKAN
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| Learning Outcomes of the Course |
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Understands gene cloning applications in biotechnology and industry
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| Mode of Delivery |
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Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
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None |
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| Aim(s) of Course |
: |
Gene cloning and apllications |
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| Course Contents |
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Why gene cloning is important, Plasmids and bacteriophages, Isolation and purification of DNA from plasmid, bacteriophages, plant cell and animal cell, Introduction of DNA into living cells (transformation, conjugation, electroporation) Cloning vectors, The polymerase chain reaction, gene cloning in microbiology, agriculture and medicine. |
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| Language of Instruction |
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Turkish |
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| Work Place |
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Labratory |
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
Importance of gene cloning |
Study in literature |
Oral presentation |
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2 |
Plasmids |
Study in literature |
Oral presentation |
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3 |
Bacteriophage |
Study in literature |
Oral presentation |
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4 |
Isolation techniques of bacterial chrommosomal DNA, Plasmid and bacteriophages nucleic acids |
Study in literature |
Oral presentation |
|
5 |
Isolation of plant chromosomal DNA techniques |
Study in literature |
Oral presentation |
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6 |
Chromosomal DNA isolation of yeast and molds |
Study in literature |
Oral presentation |
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7 |
Exam |
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exam |
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8 |
Conjugation |
Study in literature |
Oral presentation |
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9 |
Transformation |
Study in literature |
Oral presentation |
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10 |
Electroporation |
Study in literature |
Oral presentation |
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11 |
Electofusion, biolistic transformation and lipozom transformation |
Study in literature |
Oral presentation |
|
12 |
Cloning vectors |
Study in literature |
Oral presentation |
|
13 |
Production of recombinant DNA and trasferring to recipient organisms |
Study in literature |
Oral presentation |
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14 |
PCR |
Study in literature |
Oral presentation |
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15 |
Isolation of transformants |
Study in literature |
Oral presentation |
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16/17 |
Final exam |
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exam |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 Lodge, J., Lund, P., and Minchin, S. (2007). Gene Cloning.Taylor&Francis Group, 270 Madison Avenue New York, N Y 10016 USA
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| |
| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
60 |
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Homeworks/Projects/Others |
2 |
40 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
| |
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Final Assessments
|
100 |
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Rate of Final Assessments to Success
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60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Evaluates and directs his level of learning in the field of knowledge and skills with his expert level critically. |
4 |
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2 |
Transfers current developments in the field of his work, supporting them with quantitative and qualitative data, systematically to the area outside of the field, written, orally and visually. |
5 |
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3 |
Follows national and international publications and attends social interactions and scientific studies in international level, communicates in at least in one foreign language in order to share studies on international base. |
4 |
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4 |
Uses advanced information and communication technologies along with the required level of their computer software. |
4 |
|
5 |
Uses the knowledge in his field for problem solving and / or practical skills in interdisciplinary studies. |
5 |
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6 |
Improves common knowledge accumulation concerning the Biotechnology in the frame of basic theory and practices. |
5 |
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7 |
Is aware of scientific, ethical and social values and handles research process with this frame. |
5 |
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8 |
Handles theories, hypothesis, opinions in the field of Biotechnology with an objective sceptic, logical, analytical manner and evaluates them in critical point of view. |
5 |
|
9 |
Gains comprehensive information about natural and applied sciences and its limits with modern techniques and methods applied. |
5 |
|
10 |
Improves and increases the knowledge to an expert level in the field of biotechnology |
5 |
|
11 |
Understands the interdisciplinary interaction associated with biotechnology. |
5 |
|
12 |
Integrates and interprets the knowledge from different disciplines by his expertrise in biology and generate new information |
5 |
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13 |
Analizes the problems encountered in the field of research methods. |
5 |
|
14 |
Carries out a study requiring expertise in the field independently. |
5 |
|
15 |
Developes new strategic approaches and takes resposibility for analitical solutions for unpredictable complicated problems encountered in applications related to biotechnology. |
5 |
|
16 |
Demonstrates leadership in the required environment to solve problems associated with biotechnology. |
4 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
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Class Time (Exam weeks are excluded) |
14 |
2 |
28 |
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Out of Class Study (Preliminary Work, Practice) |
14 |
4 |
56 |
| Assesment Related Works |
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Homeworks, Projects, Others |
2 |
10 |
20 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
10 |
10 |
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Final Exam |
1 |
25 |
25 |
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Total Workload: | 139 |
| Total Workload / 25 (h): | 5.56 |
| ECTS Credit: | 6 |
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