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| Course Description |
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| Course Name |
: |
Analysis of Genetically Modified Organisms in Seeds and in Foods |
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| Course Code |
: |
BT-548 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
Second Cycle |
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| Year of Study |
: |
1 |
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| Course Semester |
: |
Spring (16 Weeks) |
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| ECTS |
: |
6 |
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| Name of Lecturer(s) |
: |
Prof.Dr. SALİH KAFKAS
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| Learning Outcomes of the Course |
: |
Knows sampling strategies in GMO analysis of seeds and foods
Knows DNA extraction, DNA quantification and qualification
Knows GMO screening methods by PCR
Knows quantitative and qualitative determination of GMOs by real-time PCR
Knows GMO production, importing, exporting and laws in Turkey and in the world
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| Mode of Delivery |
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Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
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| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
To teach determination of genetically modified organisms in seeds and in Foods |
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| Course Contents |
: |
Situation of GMO growing in the World, Genetically modified cultivars in the production, Transferred genes and their characteristic in GM cultivars, sampling strategies in GMO analysis, Organization of GMO laboratory, homogenization of samples, DNA extraction, quantification and qualification of DNA, GMO screening methods by PCR, Gene-specific, construct-specific and event-specific determination of GM products, qualitative and quantitattive determination of GM products by real-time PCR analysis, problems and solutions in determining of GMOs, Analysis and reporting of the results |
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| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Classrooms and Molecular Genetic Laboratory of Department of Horticulture, Faculty of Agriculture, University of Cukurova
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
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1 |
Situation of GMO growing in the World |
Reading text notes |
Text notes and related book chapters |
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2 |
Genetically modified cultivars in the production, |
Reading text notes |
Text notes and related book chapters |
|
3 |
Transferred genes and their characteristic in GM cultivars, |
Reading text notes |
Text notes and related book chapters |
|
4 |
sampling strategies in GMO analysis, |
Reading text notes |
Text notes and related book chapters |
|
5 |
Organization of GMO laboratory |
Reading text notes |
Text notes and related book chapters |
|
6 |
homogenization of samples |
Reading text notes |
Text notes and related book chapters |
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7 |
DNA extraction, quantification and qualification |
Reading text notes |
Text notes and related book chapters |
|
8 |
GMO screening methods by PCR |
Reading text notes |
Text notes and related book chapters |
|
9 |
Mid-term exam |
Reading text notes |
An exam containing the subjects studied untill the week of exam |
|
10 |
Gene-specific, construct-specific and event-specific determination of GM products |
Reading text notes |
Text notes and related book chapters |
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11 |
qualitative determination of GM products by real-time PCR analysis |
Reading text notes |
Text notes and related book chapters |
|
12 |
quantitative determination of GM products by real-time PCR analysis |
Reading text notes |
Text notes and related book chapters |
|
13 |
problems and solutions in determining of GMOs |
Reading text notes |
Text notes and related book chapters |
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14 |
Analysis and reporting of the results |
Reading text notes |
Text notes and related book chapters |
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15 |
Analysis and reporting of the results |
Reading text notes |
Text notes and related book chapters |
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16/17 |
Final exam |
Reading text notes |
Final exam containing all the studied subjects |
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Required Course Resources |
| Resource Type | Resource Name |
| Recommended Course Material(s) |
 PCR Based Methods on GMO Detection (E. Noli and C.Haldemann)
GMO analysis in Food products (M.Querci, M.Jermini and G.V. Eede)
Text notes and slides
Real-Time PCR An essential Guide (K.Edwards, J.Logan and N.Saunders)
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| Required Course Material(s) | |
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Assessment Methods and Assessment Criteria |
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Semester/Year Assessments |
Number |
Contribution Percentage |
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Mid-term Exams (Written, Oral, etc.) |
1 |
60 |
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Homeworks/Projects/Others |
3 |
40 |
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Total |
100 |
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Rate of Semester/Year Assessments to Success |
40 |
| |
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Final Assessments
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100 |
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Rate of Final Assessments to Success
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60 |
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Total |
100 |
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| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Evaluates and directs his level of learning in the field of knowledge and skills with his expert level critically. |
4 |
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2 |
Transfers current developments in the field of his work, supporting them with quantitative and qualitative data, systematically to the area outside of the field, written, orally and visually. |
5 |
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3 |
Follows national and international publications and attends social interactions and scientific studies in international level, communicates in at least in one foreign language in order to share studies on international base. |
5 |
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4 |
Uses advanced information and communication technologies along with the required level of their computer software. |
4 |
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5 |
Uses the knowledge in his field for problem solving and / or practical skills in interdisciplinary studies. |
4 |
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6 |
Improves common knowledge accumulation concerning the Biotechnology in the frame of basic theory and practices. |
5 |
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7 |
Is aware of scientific, ethical and social values and handles research process with this frame. |
4 |
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8 |
Handles theories, hypothesis, opinions in the field of Biotechnology with an objective sceptic, logical, analytical manner and evaluates them in critical point of view. |
5 |
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9 |
Gains comprehensive information about natural and applied sciences and its limits with modern techniques and methods applied. |
3 |
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10 |
Improves and increases the knowledge to an expert level in the field of biotechnology |
5 |
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11 |
Understands the interdisciplinary interaction associated with biotechnology. |
5 |
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12 |
Integrates and interprets the knowledge from different disciplines by his expertrise in biology and generate new information |
5 |
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13 |
Analizes the problems encountered in the field of research methods. |
4 |
|
14 |
Carries out a study requiring expertise in the field independently. |
5 |
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15 |
Developes new strategic approaches and takes resposibility for analitical solutions for unpredictable complicated problems encountered in applications related to biotechnology. |
5 |
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16 |
Demonstrates leadership in the required environment to solve problems associated with biotechnology. |
4 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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| Student Workload - ECTS |
| Works | Number | Time (Hour) | Total Workload (Hour) |
| Course Related Works |
|
Class Time (Exam weeks are excluded) |
14 |
4 |
56 |
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Out of Class Study (Preliminary Work, Practice) |
13 |
2 |
26 |
| Assesment Related Works |
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Homeworks, Projects, Others |
3 |
10 |
30 |
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Mid-term Exams (Written, Oral, etc.) |
1 |
14 |
14 |
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Final Exam |
1 |
14 |
14 |
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Total Workload: | 140 |
| Total Workload / 25 (h): | 5.6 |
| ECTS Credit: | 6 |
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