| Course Description |
|
| Course Name |
: |
Plant Tissue Culture |
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| Course Code |
: |
BT-517 |
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| Course Type |
: |
Optional |
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| Level of Course |
: |
Second Cycle |
|
| Year of Study |
: |
1 |
|
| Course Semester |
: |
Fall (16 Weeks) |
|
| ECTS |
: |
6 |
|
| Name of Lecturer(s) |
: |
Prof.Dr. SAADET BÜYÜKALACA
|
|
| Learning Outcomes of the Course |
: |
Obtains the conceptual and theoretical knowledge about plant tissue culture.
Knows the factors affecting the sucsess of plant tissue culture, improvement techniques and methods to increase the success of plant tissue cultures.
Knows theoretical and practical methods for different researches.
Knows to transform theoretical knowledge in laboratory studies, experiments.
Improves new methods to solve present problems.
|
|
| Mode of Delivery |
: |
Face-to-Face |
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| Prerequisites and Co-Prerequisites |
: |
None |
|
| Recommended Optional Programme Components |
: |
None |
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| Aim(s) of Course |
: |
To give general and practical information about plant tissue cultures. |
|
| Course Contents |
: |
Tissue culture-related definitions, techniques used in plant tissue culture, preparation of plant nutrient media, isolation and transferring of plant tissues, culture conditions, acclimatization of in vitro plants to environment, meristem culture, shoot tip culture, cell and callus culture. |
|
| Language of Instruction |
: |
Turkish |
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| Work Place |
: |
Post-graduate class of the Department |
|
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Course Outline /Schedule (Weekly) Planned Learning Activities |
| Week | Subject | Student's Preliminary Work | Learning Activities and Teaching Methods |
|
1 |
History of plant tissue cultures |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
2 |
Tissue culture laboratory techniques (preparation of nutrient medium, sterilization techniques of media-explant and equipment used in tissue culture, infection sources in tissue e culture) |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
3 |
Meristem culture |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
4 |
Production of disease-free plant |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
5 |
Embryo culture |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
6 |
Anther, ovule and microspore culture |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
7 |
Mid-term exam |
Preparation to mid-term exam |
Written examination |
|
8 |
Callus and cell suspension cultures |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
9 |
Direct and indirect organogenesis |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
10 |
Direct and indirect embryogenesis |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
11 |
Bioreactors and artificial seed production |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
12 |
Germplasm preservation |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
13 |
Somaclonal variations |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
14 |
Acclimatization of in vitro plants |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
15 |
General discussion about tissue culture techniques and use of these techniques in Horticulture |
Reading recommended books and given lecture notes |
lecturing, powerpoint presentation and discussion |
|
16/17 |
Final Exam |
Preparation to final exam |
Written examination |
|
|
| Contribution of the Course to Key Learning Outcomes |
| # | Key Learning Outcome | Contribution* |
|
1 |
Evaluates and directs his level of learning in the field of knowledge and skills with his expert level critically. |
2 |
|
2 |
Transfers current developments in the field of his work, supporting them with quantitative and qualitative data, systematically to the area outside of the field, written, orally and visually. |
3 |
|
3 |
Follows national and international publications and attends social interactions and scientific studies in international level, communicates in at least in one foreign language in order to share studies on international base. |
1 |
|
4 |
Uses advanced information and communication technologies along with the required level of their computer software. |
2 |
|
5 |
Uses the knowledge in his field for problem solving and / or practical skills in interdisciplinary studies. |
3 |
|
6 |
Improves common knowledge accumulation concerning the Biotechnology in the frame of basic theory and practices. |
3 |
|
7 |
Is aware of scientific, ethical and social values and handles research process with this frame. |
4 |
|
8 |
Handles theories, hypothesis, opinions in the field of Biotechnology with an objective sceptic, logical, analytical manner and evaluates them in critical point of view. |
3 |
|
9 |
Gains comprehensive information about natural and applied sciences and its limits with modern techniques and methods applied. |
5 |
|
10 |
Improves and increases the knowledge to an expert level in the field of biotechnology |
4 |
|
11 |
Understands the interdisciplinary interaction associated with biotechnology. |
2 |
|
12 |
Integrates and interprets the knowledge from different disciplines by his expertrise in biology and generate new information |
3 |
|
13 |
Analizes the problems encountered in the field of research methods. |
3 |
|
14 |
Carries out a study requiring expertise in the field independently. |
3 |
|
15 |
Developes new strategic approaches and takes resposibility for analitical solutions for unpredictable complicated problems encountered in applications related to biotechnology. |
3 |
|
16 |
Demonstrates leadership in the required environment to solve problems associated with biotechnology. |
2 |
| * Contribution levels are between 0 (not) and 5 (maximum). |
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